| Full text | |
| Author(s): |
Mendes, Luis F. S.
[1]
;
Batista, Mariana R. B.
[2, 1]
;
Kava, Emanuel
[1]
;
Bleicher, Lucas
[3]
;
Micheletto, Mariana C.
[1]
;
Costa-Filho, Antonio J.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Sch Philosophy, Phys Dept, Mol Biophys Lab, Sao Paulo - Brazil
[2] Univ Warwick, Sch Life Sci, Gibbet Hill Campus, Coventry, W Midlands - England
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Belo Horizonte, MG - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 194, p. 264-275, JAN 1 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
The Golgi complex is an essential organelle of the eukaryotic exocytic pathway. A subfamily of Golgi matrix proteins, called GRASPs, is central in stress-induced unconventional secretion, Golgi dynamics during mitosis/ apoptosis, and Golgi ribbon formation. The Golgi ribbon is vertebrate-specific and correlates with the appearance of two GRASP paralogues and two Golgins (GM130/Golgin45), which form specific GRASP-Golgin pairs. The molecular details of their appearance only in Metazoans are unknown. Moreover, despite new functionalities supported by GRASP paralogy, little is known about their structural and evolutionary differences. Here, we used ancestor sequence reconstruction and biophysical/biochemical approaches to assess the evolution of GRASPs structure/dynamics, fibrillation, and how they started anchoring their Golgin partners. Our data showed that a GRASP ancestor anchored Golgins before gorasp gene duplication in Metazoans. After gene duplication, variations within the GRASP binding pocket determined which paralogue would recruit which Golgin. These interactions are responsible for their specific Golgi location and Golgi ribbon appearance. We also suggest that GRASPs have a long-standing capacity to form supramolecular structures, affecting their participation in stress induced processes. (AU) | |
| FAPESP's process: | 16/16328-3 - Molecular dynamics simulations of proton dependent oligopeptide transporters |
| Grantee: | Mariana Raquel Bunoro Batista |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/16812-0 - Multi-User Equipment approved in grant 2014/15546-1: SEC-MALS |
| Grantee: | Richard Charles Garratt |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 15/50366-7 - Resolving mechanistic details of peptide transport across membranes using crystallographic and non-crystallographic structural biology approaches |
| Grantee: | Antonio José da Costa Filho |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/20367-3 - Structural and functional studies of the Golgi Re-Assembly and Stacking Protein (GRASP) from Cryptococcus neoformans |
| Grantee: | Antonio José da Costa Filho |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/13016-6 - Activation of Photosensitive Proteins with Radioluminescent Nanoparticles and X-Rays |
| Grantee: | Mariana Chaves Micheletto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/24669-8 - Unraveling the molecular bases of the early protein secretory pathway in humans using biophysical techniques |
| Grantee: | Luis Felipe Santos Mendes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |