Cultivation of Chlamydomonas reinhardtii in Anaerobically Digested Vinasse for Bioethanol Production

Author(s)

Tasic, Marija B. ^[1] ; Bonon, Anderson de Jesus ^[2] ; Rocha Barbosa Schiavon, Maria Ingrid ^[3] ; Klein, Bruno Colling ^[4] ; Veljkovic, Vlada B. ^{[1, 5]} ; Maciel Filho, Rubens ^[3]

Affiliation

^[1] Univ Nis, Fac Technol, Bulevar Oslobodjenja 124, Leskovac 16000 - Serbia

^[2] HORIBA Instruments Inc, HORIBA Sci, 9755 Res Dr, Irvine, CA 92618 - USA

^[3] Univ Estadual Campinas, Fac Chem Engn, Univ Town Zeferino Vaz, Ave Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

^[4] Natl Renewable Energy Lab, 15013 Denver W Pkwy, Golden, CO 80401 - USA

^[5] Serbian Acad Arts & Sci, Knez Mihailova 35, Belgrade 11000 - Serbia

• Document type: Journal article
• Source: WASTE AND BIOMASS VALORIZATION; v. 12, n. 2, SI, p. 857-865, FEB 2021.
• Web of Science Citations: 4

Abstract

This study describes a new algal biofuel process that integrates sugarcane biorefinery wastewater treatment by nutrient removal with algae into bioethanol. The process is free of common industrial problems, including algal contamination, nutrients and fresh water usage, carbohydrate extraction, liquefaction, and saccharification. Cultivation and fermentation were conducted in one step by turning the light-air on and off, respectively. Three series of experiments with Chlamydomonas reinhardtii CC-1093 cultivation and fermentation were performed in anaerobically digested vinasse. Control experiments were a reference to compare the influence of chloride and ammonium-sulfate stress conditions on ethanol yield. Experimental results showed: (1) algal biomass can be successfully cultured within biorefinery wastewater (1129 mg center dot L-1 center dot day(-1)); (2) relatively high bioremediation was achieved (26.1%-83.5%); (3) obtained ethanol yield was (maximum 68.3% of the theoretical yield) in one process step; and (4) the chloride stress condition influences on algae to synthesize extracellular polysaccharides as add-in product (120 mg/L). {[}GRAPHICS] . (AU)

• FAPESP's process: 17/14056-9 - Chlamydomonas reinhardtii cultivation in vinasse for bioethanol production
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants