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Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest

Abstract

The biopharmaceuticals' production and commercialization are directly related to the process scale-up and cost for the entire process up to the final product. This is especially true for complex molecules that are already difficult to produce by using the currently available commercial production platforms. The mammalian cell culture technology, even though produces primarily over 130 commercial therapeutic proteins, it is an expensive production platform due to its complex growth requirements, as well as low protein expression. As the other photosynthetic microorganisms, Chlamydomonas reinhardtii, growth requirements are much simpler and cheaper (salts, light and carbon dioxide). Some of the features for Chlamydomonas as recombinant proteins production platform include: fast generation of stable transformed cell lines, efficient protein folding in non-reducing environment, cell doubling time in about 10h in inorganic media and scalability. The objective of this research is to use Chlamydomonas reinhardtii as a production platform for biopharmaceuticals including some of the ones presented, in 2015, by SUS (Sistema Único de Saúde - Brazilian Public Health System) as products' priorities for Brazil. Proteins with different complexity, expression, accumulation and activity analyses will be evaluated through the techniques of Molecular Biology, western blotting, biomolecules purification, ELISA and cell proliferation assays. The recombinant strains will also be cultivated in a tubular photobioreactor using different light intensities with the purpose of kinetics parameters evaluation, as well as the recombinant protein accumulation during the process cultivation. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ARIAS, CESAR ANDRES DIAZ; MATSUDO, MARCELO CHUEI; FERREIRA-CAMARGO, LIVIA SENO; MOLINO, JOAO VITOR DUTRA; MAYFIELD, STEPHEN PATRICK; DE CARVALHO, JOAO CARLOS MONTEIRO. Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii. BIOTECHNOLOGY PROGRESS, v. 37, n. 2, . (17/24486-0, 16/12992-6)
PESSOA, JASSIARA DA SILVA; DE OLIVEIRA, CAROLINE FRERE MARTINIUC; MENA-CHALCO, JESUS PASCUAL; DE CARVALHO, JOAO CARLOS MONTEIRO; FERREIRA-CAMARGO, LIVIA SENO. Trends on Chlamydomonas reinhardtii growth regimes and bioproducts. Biotechnology and Applied Biochemistry, v. N/A, p. 13-pg., . (19/19308-1, 16/12992-6, 19/13822-5)

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