| Full text | |
| Author(s): |
Arias, Cesar Andres Diaz
[1]
;
Matsudo, Marcelo Chuei
[2]
;
Ferreira-Camargo, Livia Seno
[3]
;
Molino, Joao Vitor Dutra
[1]
;
Mayfield, Stephen Patrick
[4, 5]
;
de Carvalho, Joao Carlos Monteiro
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil
[2] Univ Fed Itajuba, Inst Nat Resources, Itajuba, MG - Brazil
[3] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil
[4] Univ Calif San Diego, Dept Mol Biol, San Diego, CA 92103 - USA
[5] Univ Calif San Diego, Calif Ctr Algae Biotechnol, San Diego, CA 92103 - USA
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BIOTECHNOLOGY PROGRESS; v. 37, n. 2 NOV 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe (GRAS) by the Food and Drug Administration (FDA). Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product productivity, when compared to batch process. As there is a lack of studies on semicontinuous process for recombinant protein production in microalgae, the production of recombinant mCherry fluorescent protein was evaluated by semicontinuous cultivation of Chlamydomonas reinhardtii in bubble column photobioreactor. This semicontinuous cultivation process was evaluated in the following conditions: 20%, 40%, and 60% culture portion withdrawal. The highest culture withdrawal percentage (60%) provided the best results, as an up to 161% increase in mCherry productivity (454.5 RFU h(-1) - Relative Fluorescence Unit h(-1)), in comparison to batch cultivation (174.0 RFU h(-1)) of the same strain. All cultivations were carried out for 13 days, at pH 7, temperature 25 degrees C and, by semicontinuous process, two culture withdrawals were taken during the cultivations. Throughout the production cycles, it was possible to obtain biomass concentration up to 1.36 g L-1. (AU) | |
| FAPESP's process: | 17/24486-0 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest |
| Grantee: | Lívia Seno Ferreira Camargo |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 16/12992-6 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest |
| Grantee: | Lívia Seno Ferreira Camargo |
| Support Opportunities: | Research Grants - Young Investigators Grants |