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Recombinant Somatotropin produced in Chlamydomonas reinhardtii

Grant number: 19/13822-5
Support Opportunities:Scholarships in Brazil - Master
Start date: August 01, 2019
End date: December 31, 2021
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Lívia Seno Ferreira Camargo
Grantee:Jassiara da Silva Pessoa
Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
Associated research grant:16/12992-6 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest, AP.JP

Abstract

The use of Chlamydomonas reinhardtii to accumulate recombinant proteins was already demonstrated. However, besides the need for this production platform improvement, some proteins were not still evaluated to be expressed and accumulated in specific cell organelles. Somatotropin is a hormonal peptide synthesized and secreted by the anterior pituitary in humans and other vertebrates, and it stimulates growth, cell reproduction and regeneration in humans and animals. This biological drug is consumed by people with this hormone deficiency and that present, mainly, short stature. In order to verify the possibility of using the microalgae Chlamydomonas reinhardtii as a low cost platform for the expression of the protein somatotropin, microalgae chloroplasts will be genetically transformed by the biobalistics technique. The expression, accumulation and analysis of the molecule will be evaluated through PCR techniques and western blotting.

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
PESSOA, JASSIARA DA SILVA; DA SILVA, BRUNO GUZZO; DE FRANCA JR, EDNILSON DONISETE; DA SILVA FILHO, ISAC JOSE; MOLINO, JOAO VITOR DUTRA; DE CARVALHO, JOAO CARLOS MONTEIRO; FERREIRA-CAMARGO, LIVIA SENO. Cultivation Strategies to Improve Chlamydomonas reinhardtii Growth and Recombinant Mcherry Expression. JOURNAL OF BASIC MICROBIOLOGY, v. N/A, p. 8-pg., . (16/12992-6, 19/13822-5)
PESSOA, JASSIARA DA SILVA; DE OLIVEIRA, CAROLINE FRERE MARTINIUC; MENA-CHALCO, JESUS PASCUAL; DE CARVALHO, JOAO CARLOS MONTEIRO; FERREIRA-CAMARGO, LIVIA SENO. Trends on Chlamydomonas reinhardtii growth regimes and bioproducts. Biotechnology and Applied Biochemistry, v. N/A, p. 13-pg., . (19/19308-1, 16/12992-6, 19/13822-5)