Therapeutic recombinant protein accumulation in Chlamydomonas reinhardtii

• Grant number: 21/09747-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2021
• Effective date (End): January 31, 2023
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Lívia Seno Ferreira Camargo
• Grantee: Ednilson Donisete de França Junior
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated research grant: 16/12992-6 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest, AP.JP

Abstract

The use of Chlamydomonas reinhardtii for the accumulation of recombinant proteins has already been demonstrated by different researchers working with microalgal genetic engineering, however, there are proteins that have not yet been tested. Among them are some biological products that were, in 2015, considered as some of the priority medicines for the SUS (Unified Health System) in Brazil. In order to verify the possibility of using the microalga Chlamydomonas reinhardtii for protein expression: infliximab, adalimumab, somatotropin, and filgrastim, microalgae chloroplasts will be genetically transformed by the biolistic technique. The expression, accumulation, and analysis of the molecule will be evaluated using PCR, western blotting techniques. (AU)