Evaluation of Chlamydomonas reinhardtii as a production platform of recombinant filgrastime protein

• Grant number: 19/19308-1
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): October 01, 2019
• Effective date (End): September 30, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Lívia Seno Ferreira Camargo
• Grantee: Caroline Frere Martiniuc de Oliveira
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated research grant: 16/12992-6 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest, AP.JP

Abstract

Chlamydomonas reinhardtii has been studied and used for accumulation of recombinant proteins. There are several therapeutic proteins of national interest that can be produced on this low cost microalgae platform. Filgrastime is a biological medicine that resembles the natural G-CSF (Granulocytic Colony-Stimulating Factor) of the human organism, acting to reduce the chances of infection in people during treatment of certain cancers, who have had bone marrow transplant or have chronic neutropenia, as it contributes to granulocyte proliferation and differentiation. In order to verify the possibility of using Chlamydomonas reinhardtii microalgae for filgrastime expression, microalgae chloroplasts will be genetically transformed by the biobalistic technique. The expression, accumulation and analysis of the molecule will be evaluated by PCR techniques, western blotting. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PESSOA, JASSIARA DA SILVA; DE OLIVEIRA, CAROLINE FRERE MARTINIUC; MENA-CHALCO, JESUS PASCUAL; DE CARVALHO, JOAO CARLOS MONTEIRO; FERREIRA-CAMARGO, LIVIA SENO. Trends on Chlamydomonas reinhardtii growth regimes and bioproducts. Biotechnology and Applied Biochemistry, v. N/A, p. 13-pg., 2023-06-19. (19/19308-1, 16/12992-6, 19/13822-5)