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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of Moquiniastrum polymorphum (Asteraceae)

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Author(s):
Farinella, Victor F. [1] ; Kawafune, Eunizinis S. [1] ; Tangerina, Marcelo M. P. [1] ; Domingos, Helori V. [2] ; Costa-Lotufo, Leticia V. [2] ; Ferreira, Marcelo J. P. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Molecules; v. 26, n. 23 DEC 2021.
Web of Science Citations: 0
Abstract

Three endophytic fungi isolated from Moquiniastrum polymorphum (Less.) G. Sancho (Asteraceae) were cultivated using the one strain many compounds (OSMAC) strategy to evaluate the production of griseofulvin derivatives. Extracts obtained were analyzed by HPLC-MS/MS and the chromatographic and spectrometric data used to elaborate a feature-based molecular network (FBMN) through the GNPS platform. This approach allowed the observation of differences such as medium-specific and strain-specific production of griseofulvin derivatives and variations of cytotoxic activity in most extracts. To evaluate the efficiency of the OSMAC approach allied with FBMN analysis in the prospection of compounds of biotechnological interest, griseofulvin and 7-dechlorogriseofulvin were isolated, and the relative concentrations were estimated in all culture media using HPLC-UV, allowing for the inference of the best strain-medium combinations to maximize its production. Malt extract-peptone broth and Wickerham broth media produced the highest concentrations of both secondary metabolites. (AU)

FAPESP's process: 20/16554-9 - Asteraceae and endophytic fungi: identification of natural products and biological activities
Grantee:Marcelo José Pena Ferreira
Support Opportunities: Regular Research Grants
FAPESP's process: 15/17177-6 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds
Grantee:Leticia Veras Costa Lotufo
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants