nantiomer-dependent immunological response to chiral nanoparticle

Author(s):Show less -

Xu, Liguang ^{[1, 2]} ; Wang, Xiuxiu ^{[1, 2]} ; Wang, Weiwei ^{[1, 2]} ; Sun, Maozhong ^{[1, 2]} ; Choi, Won Jin ^[3] ; Kim, Ji-Young ^[4] ; Hao, Changlong ^{[1, 2]} ; Li, Si ^[5] ; Qu, Aihua ^{[1, 2]} ; Lu, Meiru ^{[1, 2]} ; Wu, Xiaoling ^{[1, 2]} ; Colombari, Felippe M. ^[6] ; Gomes, Weverson R. ^[7] ; Blanco, Asdrubal L. ^[7] ; de Moura, Andre F. ^[7] ; Guo, Xiao ^{[1, 2]} ; Kuang, Hua ^{[1, 2, 5, 8]} ; Kotov, Nicholas A. ^{[3, 4]} ; Xu, Chuanlai ^{[1, 2]}

Affiliation

^[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu - Peoples R China

^[2] Jiangnan Univ, Sch Food Sci & Technol, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu - Peoples R China

^[3] Univ Michigan, Dept Mat Sci & Engn, Ann Arbor, MI 48109 - USA

^[4] Univ Michigan, Dept Chem Engn, Biointerface Inst, Ann Arbor, MI 48109 - USA

^[5] Jiangnan Univ, Sch Chem & Mat Engn, Key Lab Synthet & Biol Colloids, Minist Educ, Wuxi, Jiangsu - Peoples R China

^[6] Brazilian Ctr Res Energy & Mat, Brazilian Nanotechnol Natl Lab, Campinas, SP - Brazil

^[7] Univ Fed Sao Carlos, Dept Chem, Sao Carlos, SP - Brazil

^[8] Jiangnan Univ, Sci Ctr Future Foods, Wuxi, Jiangsu - Peoples R China

• Document type: Journal article
• Source: Nature; v. 601, n. 7893, p. 366+, JAN 20 2022.
• Web of Science Citations: 1

Abstract

Chirality is a unifying structural metric of biological and abiological forms of matter. Over the past decade, considerable clarity has been achieved in understanding the chemistry and physics of chiral inorganic nanoparticles(1-4); however, little is known about their effects on complex biochemical networks(5,6). Intermolecular interactions of biological molecules and inorganic nanoparticles show some commonalities(7-9), but these structures differ in scale, in geometry and in the dynamics of chiral shapes, which can both impede and strengthen their mirror-asymmetric complexes. Here we show that achiral and left- and right-handed gold biomimetic nanoparticles show different in vitro and in vivo immune responses. We use irradiation with circularly polarized light (CPL) to synthesize nanoparticles with controllable nanometre-scale chirality and optical anisotropy factors (g-factors) of up to 0.4. We find that binding of nanoparticles to two proteins from the family of adhesion G-protein-coupled receptors (AGPCRs)-namely cluster-of-differentiation 97 (CD97) and epidermal-growth-factor-like-module receptor 1 (EMR1)-results in the opening of mechanosensitive potassium-efflux channels, the production of immune signalling complexes known as inflammasomes, and the maturation of mouse bone-marrow-derived dendritic cells. Both in vivo and in vitro immune responses depend monotonically on the g-factors of the nanoparticles, indicating that nanoscale chirality can be used to regulate the maturation of immune cells. Finally, left-handed nanoparticles show substantially higher (1,258-fold) efficiency compared with their right-handed counterparts as adjuvants for vaccination against the H9N2 influenza virus, opening a path to the use of nanoscale chirality in immunology. (AU)

• FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
• Grantee: Elson Longo da Silva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 12/15147-4 - Computational study of thermodynamic association of self­-assembled systems
• Grantee: André Farias de Moura
• Support Opportunities: Regular Research Grants