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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

evelopment, validation, and application of an HPLC-MS/MS method for quantification of oxidized fatty acids in plant

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Author(s):
Almeida-Trapp, Marilia [1, 2, 3] ; de Souza, Gezimar Donizetti [4] ; Shekhawat, Kirti [3] ; Sheikh, Arsheed H. [3] ; Mithofer, Axel [2] ; Hirt, Heribert [3] ; Rodrigues-Filho, Edson [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Carlos, LaBioMMi, Sao Carlos, SP - Brazil
[2] Max Planck Inst Chem Ecol, Jena - Germany
[3] King Abdullah Univ Sci & Technol, Thuwal - Saudi Arabia
[4] Accert Chem & Biotechnol Inc, Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1186, DEC 1 2021.
Web of Science Citations: 0
Abstract

Oxylipins constitute a huge class of compounds produced by oxidation of long-chain unsaturated fatty acids either chemically (by radicals such as reactive oxygen species, ROS) or enzymatically (by lipoxygenases, LOX; cyclooxygenases, COX; or cytochrome P450 pathways). This process generates fatty acids peroxides, which can then be further modified in a broad range to epoxy, hydroxy, keto, ether fatty acids, and also hydrolyzed to generate small aldehydes and alcohols. In general, oxylipins are present in almost all living organisms and have a wide range of signaling, metabolic, physiological, and ecological roles depending on the particular organism and on their structure. In plants, oxylipins have been extensively studied over the past 35 years. However, these studies have focused mainly on the jasmonates and so-called green leaves volatiles. The function of early LOX products (like keto and hydroxy fatty acids) is yet not well understood in plants, where they are mainly analyzed by indirect methods or by GC-MS what requires a laborious sample preparation. Here, we developed and validated a straightforward, precise, accurate, and sensitive method for quantifying oxylipins in plant tissues using HPLC-MS/MS, with a one-step extraction procedure using low amount of plant tissues. We successfully applied this method to quantify the oxylipins in different plant species and Arabidopsis thaliana plants treated with various biotic and abiotic stress conditions. (AU)

FAPESP's process: 10/17925-9 - "BIOACTIVITY, CHEMICAL AND MICROBIAL DIVERSITY IN ALTERNANTHERA SPECIES"
Grantee:Marília Almeida Trapp
Support Opportunities: Scholarships in Brazil - Doctorate