he association of Edaravone with shunt surgery improves behavioral performance, reduces astrocyte reaction and apoptosis, and promotes neuroprotection in young hydrocephalic rat

Author(s)

Romeiro, Thais Helena ^[1] ; Da Silva, Stephanya Covas ^[1] ; Beggiora, Pamella da Silva ^[1] ; Sampaio, Gustavo Botelho ^[2] ; Brandao, Ricardo Andrade ^[3] ; Santos, Marcelo Volpon ^[1] ; Machado, Helio Rubens ^[1] ; Lopes, Luiza da Silva ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Bandeirantes Av 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Childrens & Matern Hosp Sao Jose do Rio Preto, Dept Pediat Neurosurg, Jamil Ferreira Kfouri Av 3355, BR-15091240 Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Neurosci & Behav, Bandeirantes Av 3900, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of Chemical Neuroanatomy; v. 119, JAN 2022.
• Web of Science Citations: 0

Abstract

The neuroprotective effect of Edaravone in young hydrocephalic rats associated with a CSF derivation system was evaluated. The drug has already been shown to be beneficial in experimental hydrocephalus, but the combination of this drug with shunt surgery has not yet been investigated. Fifty-seven-day-old Wistar rats submitted to hydrocephalus by injection of kaolin in the cisterna magna were used and divided into five groups: control (n = 10), hydrocephalic (n = 10), hydrocephalic treated with Edaravone (20 mg/kg/day) (n = 10), hydrocephalic treated with shunt (n = 10) and hydrocephalic treated with shunt and Edaravone (n = 10). Administration of the Edaravone was started 24 h after hydrocephalus induction (P1) and continued until the experimental endpoint (P21). The CSF shunt surgery was performed seven days after hydrocephalus induction (P7). Open-field tests, histological evaluation by hematoxylin and eosin, immunohistochemistry by Caspase-3 and GFAP, and ELISA biochemistry by GFAP were performed. Edaravone reduced reactive astmgliosis in the corpus callosum and germinal matrix (p < 0.05). When used alone or associated with CSF shunt surgery, the drug decreased the cell death process (p < 0.0001) and improved the morphological aspect of the astmglia (p < 0.05). The results showed that Edaravone associated with CSF bypass surgery promotes neuroprotection in young hydrocephalic rats by reducing reactive astrogliosis and decreasing cell death. (AU)

• FAPESP's process: 16/11212-7 - Role of hyperbaric oxygen therapy in the control of brain lesions in experimental hydrocephalus
• Grantee: Luiza da Silva Lopes
• Support Opportunities: Regular Research Grants