he Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophil

Author(s):Show less -

Reis, Ana Paula Carvalho ^[1] ; Celestrino, Giovanna Azevedo ^{[1, 2]} ; Igoa, Mariana Villas Boas ^[1] ; Jesus, Thais Martins ^[1] ; Franca, Tabata Takahashi ^[3] ; Moreira, Daniel Valerio Silva ^[1] ; Rigato, Paula Ordonhez ^[4] ; Sato, Paula Keiko ^[5] ; Condino-Neto, Antonio ^[3] ; Noronha, Irene L. ^[2] ; Dias-Melicio, Luciane Alarcao ^[6] ; Lalwani, Pritesh Jaychand ^[7] ; Benard, Gil ^[1] ; Sousa, Maria Gloria Teixeira ^[1]

Affiliation

^[1] Univ Sao Paulo, Lab Med Mycol LIM 53, Clin Dermatol Div, Hosp Clin FMUSP, Fac Med FMUSP, Inst Trop Med, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Cellular Genet & Mol Nephrol, Div Nephrol, Sch Med, BR-05403000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05403000 Sao Paulo - Brazil

^[4] Adolfo Lutz Inst, Ctr Imunol, BR-01246000 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Lab Med Investigat Immunol LIM 48, Hosp Clin HCFMUSP, Fac Med, BR-05403000 Sao Paulo - Brazil

^[6] UNESP Sao Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18618687 Botucatu, SP - Brazil

^[7] Fiocruz Amazonia, Leonidas & Maria Deane Inst, BR-69057070 Manaus, Amazonas - Brazil

• Document type: Journal article
• Source: OURNAL OF FUNG; v. 8, n. 2 FEB 2022.
• Web of Science Citations: 0

Abstract

Neutrophils are the first leukocytes recruited to the site of infection and are thought to be responsible for fungal elimination from the skin such as dermatophytes. Neutrophils are able to secrete reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) that can kill different fungi, including Aspergillus, spp., Candida albicans, and Phialophora verrucosa. However, NET production in response to Trichophyton rubrum, the main etiologic agent of dermatophytosis, has yet to be studied. We demonstrated that human neutrophils produce NETs against different morphotypes of T. rubrum in a dose-dependent manner and NET formation is dependent on ROS production. In addition, ROS production by human neutrophils in response to T. rubrum is dependent on NADPH oxidase, but not on fungal viability. NETs mediated killing of T. rubrum. Collectively, these results demonstrate that T. rubrum was able to trigger the production of NETs, suggesting that these extracellular structures may represent an important innate immune effector mechanism controlling physiological response to T. rubrum infection. (AU)

• FAPESP's process: 17/26208-8 - Chronic disseminated dermatophytosis due to Trichophyton rubrum: role of neutrophils in the effector mechanisms and in the modulation of the adaptive response of the human infection
• Grantee: Maria da Gloria Sousa Stafocker
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 16/16369-1 - Chronic disseminated dermatophytosis due to Trichophyton rubrum: role of neutrophils in the effector mechanisms and in the modulation of the adaptive response of the human infection
• Grantee: Maria da Gloria Sousa Stafocker
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/24175-8 - In situ immune response in human disseminated chronic dermatophytosis: participation of c type lectin receptors
• Grantee: Ana Paula Carvalho dos Reis
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/16146-8 - Chronic disseminated dermatophytosis due to Trichophyton rubrum: role of neutrophils in the effector mechanisms and in the modulation of the adaptive response of the human infection
• Grantee: Giovanna Azevedo Celestrino
• Support Opportunities: Scholarships in Brazil - Master