dvanced Microfluidic Technologies for Lipid Nano-Microsystems from Synthesis to Biological Applicatio

Author(s)

Carvalho, Bruna G. ^[1] ; Ceccato, Bruno T. ^[1] ; Michelon, Mariano ^[2] ; Han, Sang W. ^[3] ; de la Torre, Lucimara G. ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Chem Engn, Dept Mat & Bioproc Engn, BR-13083852 Campinas - Brazil

^[2] Fed Univ Rio Grande FURG, Sch Chem & Food Engn, BR-96203900 Rio Grande - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Dept Biophys, Ctr Cell Therapy & Mol, BR-04044010 Sao Paulo - Brazil

• Document type: Journal article
• Source: HARMACEUTIC; v. 14, n. 1 JAN 2022.
• Web of Science Citations: 0

Abstract

Microfluidics is an emerging technology that can be employed as a powerful tool for designing lipid nano-microsized structures for biological applications. Those lipid structures can be used as carrying vehicles for a wide range of drugs and genetic materials. Microfluidic technology also allows the design of sustainable processes with less financial demand, while it can be scaled up using parallelization to increase production. From this perspective, this article reviews the recent advances in the synthesis of lipid-based nanostructures through microfluidics (liposomes, lipoplexes, lipid nanoparticles, core-shell nanoparticles, and biomimetic nanovesicles). Besides that, this review describes the recent microfluidic approaches to produce lipid micro-sized structures as giant unilamellar vesicles. New strategies are also described for the controlled release of the lipid payloads using microgels and droplet-based microfluidics. To address the importance of microfluidics for lipid-nanoparticle screening, an overview of how microfluidic systems can be used to mimic the cellular environment is also presented. Future trends and perspectives in designing novel nano and micro scales are also discussed herein. (AU)

• FAPESP's process: 15/20206-8 - Modulation of monocytes, macrophages and pericytes by the colony stimulating factor genes to treat murine limb ischemia
• Grantee: Sang Won Han
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/18523-3 - Polymeric microparticle synthesis via droplet microfluidics for sustained release of non-viral vectors applied to gene therapy
• Grantee: Bruna Gregatti de Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/19537-8 - MICROFLUIDICS AS A TECHNOLOGICAL PLATFORM FOR NANO & BIOTECHNOLOGY
• Grantee: Lucimara Gaziola de la Torre
• Support Opportunities: Regular Research Grants