| Full text | |
| Author(s): Show less - |
Vitor Loureiro da Silva
;
Sérgio Luiz Borges de Souza
[2]
;
Gustavo Augusto Ferreira Mota
[3]
;
Dijon H. S. Campos
[4]
;
Alexandre Barroso Melo
[5]
;
Danielle Fernandes Vileigas
[6]
;
Paula Grippa SantAna
[7]
;
Priscila Murucci Coelho
[8]
;
Silméia Garcia Zanati Bazan
[9]
;
André Soares Leopoldo
[10]
;
Antônio Carlos Cicogna
[11]
Total Authors: 11
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Cardiologia; v. 118, n. 2, p. 463-475, 2022-02-28. |
| Abstract | |
Abstract Background Maladaptive cardiac remodelling is characterized by diastolic and systolic dysfunction, culminating in heart failure. In this context, the dysfunctional scenario of cardiac calcium (Ca2+) handling has been poorly studied. An experimental model of aortic stenosis has been extensively used to improve knowledge about the key mechanisms of cardiac pathologic remodelling. Objective To understand the dysfunctional process of the major components responsible for Ca2+ balance and its influence on cardiac function in heart failure induced by aortic stenosis. Methods Male 21-day-old Wistar rats were distributed into two groups: control (sham; n= 28) and aortic stenosis (AoS; n= 18). Cardiac function was analysed by echocardiogram, isolated papillary muscle, and isolated cardiomyocytes. In the papillary muscle assay, SERCA2a and L-type Ca2+ channel activity was evaluated. The isolated cardiomyocyte assay evaluated Ca2+ handling. Ca2+ handling protein expression was analysed by western blot. Statistical significance was set at p <0.05. Results Papillary muscles and cardiomyocytes from AoS hearts displayed mechanical malfunction. AoS rats presented a slower time to the Ca2+ peak, reduced Ca2+ myofilament sensitivity, impaired sarcoplasmic reticulum Ca2+ influx and reuptake ability, and SERCA2a and L-type calcium channel (LTCC) dysfunction. Moreover, AoS animals presented increased expression of SERCA2a, LTCCs, and the Na+/Ca2+ exchanger. Conclusion Systolic and diastolic heart failure due to supravalvular aortic stenosis was paralleled by impairment of cellular Ca2+ influx and inhibition of sarcoplasmic reticulum Ca2+ reuptake due to LTCC and SERCA2a dysfunction, as well as changes in Ca2+ handling and expression of the major proteins responsible for cellular Ca2+ homeostasis. (AU) | |
| FAPESP's process: | 15/20013-5 - Participation of calcium transient and their regulatory proteins in cardiac function improvements in rats with supravalvar aortic stenosis and ventricular dysfunction submitted to exercise training |
| Grantee: | Vitor Loureiro da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |