Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas

Author(s)

Raquel Lozano Guilharducci ^[1] ; Pedro Luiz Porfirio Xavier ^[2] ; Juliano Coelho da Silveira ^[3] ; Yonara de Gouveia Cordeiro ^[4] ; Luiz Roberto Biondi ^[5] ; Ricardo de Francisco Strefezzi ^[6] ; Heidge Fukumasu ^[7]

Affiliation

^[1] Universidade de São Paulo (USP). Laboratório de Oncologia Comparada e Translacional. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos (FZEA) - Brasil

^[2] Universidade de São Paulo (USP). Laboratório de Oncologia Comparada e Translacional. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos (FZEA) - Brasil

^[3] Universidade de São Paulo (USP). Laboratório de Morfofisiologia Molecular e Desenvolvimento. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos (FZEA) - Brasil

^[4] Universidade de São Paulo (USP). Laboratório de Oncologia Comparada e Translacional. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos (FZEA) - Brasil

^[5] Universidade Metropolitana de Santos (UNIMES). Faculdade de Medicina Veterinária - Brasil

^[6] Universidade de São Paulo (USP). Laboratório de Oncologia Comparada e Translacional. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos (FZEA) - Brasil

^[7] Universidade de São Paulo (USP). Laboratório de Oncologia Comparada e Translacional. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos (FZEA) - Brasil

• Document type: Journal article
• Source: Ciência Rural; v. 52, n. 10 2022-03-14.

Abstract

ABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas. (AU)

• FAPESP's process: 17/11966-4 - Epigenetic modulations and self-renewal potential of canine mammary cancer cells: the search for potential therapeutic targets
• Grantee: Pedro Luiz Porfirio Xavier
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/02493-7 - Mammary tumors of dogs and the cancer stem cell theory: a comparative and translational approach
• Grantee: Heidge Fukumasu
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/05778-6 - Combined inhibition of BET proteins AND HDACs as an effective strategy for the treatment of mammary cancer: a comparative study
• Grantee: Pedro Luiz Porfirio Xavier
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/10036-8 - Assessment of Lin28a/b>let-7 pathway in cell culture isolated from canine mammary carcinoma
• Grantee: Raquel Lozano Guilharducci
• Support Opportunities: Scholarships in Brazil - Scientific Initiation