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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Calcium silicate-based cements affect the cell viability and the release of TGF-β1 from apical papilla cells

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Author(s):
Marlus da Silva Pedrosa [1] ; Fernando Neves Nogueira [2] ; Carla Renata Sipert [3]
Total Authors: 3
Affiliation:
[1] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil
[2] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil
[3] University of São Paulo. School of Dentistry - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Dental Journal; v. 32, n. 6, p. 1-7, 2022-01-05.
Abstract

Abstract This study investigated the cytotoxicity and release of Transforming Growth Factor Beta 1 (TGF-β1) from cultured human apical papilla cells (APCs) after application of four bioactive materials. Culture of APCs was established and used for cytotoxic and quantitative assays. Extracts of Biodentine, Bio-C Repair, MTA Repair and White MTA were prepared and diluted (1, 1:4 and 1:16) and used for MTT assays up to 72 h. Total TGF-β1 was quantified by ELISA. Data were analyzed by ANOVA and Tukey’s test (α = 0.05). For Biodentine, at 24 h and 48 h, cell viability was lower than control (p < 0.05). At 72 h, only undiluted extract of Biodentine were cytotoxic (p < 0.05). At 24 h, a cytotoxic effect was found for undiluted and 1:4 dilution of Bio-C Repair (p < 0.05). At 48 h, however, Bio-C Repair at 1:4 and 1:8 dilution showed higher cell viability (p < 0.05). At 24 and 48 h, the cell viability for undiluted MTA Repair were higher than control (p < 0.05). For White MTA, at 24 and 48 h, all dilutions were cytotoxic (p < 0.05). All cements led to reduced release of total TGF-β1 from the APCs (p < 0.05). In conclusion, cell viability varied depending on the material and dilution. Only Bio-C repair and MTA repair led to higher cell viability of APCs. All materials induced a decrease in the release of total TGF-β1 from the APCs. (AU)

FAPESP's process: 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study
Grantee:Carla Renata Sipert
Support Opportunities: Regular Research Grants