Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

Author(s)

Gabriela Kinue Watase Kunii ^[1] ; Rossana Falcone ^[2] ; Leandro da Costa Clementino ^[3] ; João Aristeu da Rosa ^[4] ; Juliana Damieli Nascimento ^[5] ; Tiago Belintani ^[6] ; Jader de Oliveira ^[7] ; Aline Rimoldi Ribeiro ^[8]

Affiliation

^[1] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Farmacêuticas - Brasil

^[2] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Farmacêuticas - Brasil

^[3] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Farmacêuticas - Brasil

^[4] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Farmacêuticas - Brasil

^[5] Universidade Estadual de Campinas. Departamento de Biologia Animal - Brasil

^[6] Universidade Estadual de Campinas. Departamento de Biologia Animal - Brasil

^[7] Universidade de São Paulo. Departamento de Epidemiologia - Brasil

^[8] Universidade Estadual de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia - Brasil

• Document type: Journal article
• Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 55, 2022-04-08.

Abstract

ABSTRACT Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems. (AU)

• FAPESP's process: 18/25413-0 - Biological and molecular characterization of two strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae)
• Grantee: Gabriela Kinue Watase Kunii
• Support Opportunities: Scholarships in Brazil - Scientific Initiation