| Full text | |
| Author(s): Show less - |
Jarduli-Maciel, Luciana Ribeiro
;
Cottas de Azevedo, Julia Teixeira
;
Clave, Emmanuel
;
de Mello Costa, Thalita Cristina
;
Marliere Arruda, Lucas Coelho
;
Fournier, Isabelle
;
Bonini Palma, Patricia Vianna
;
Lima, Keli Cristina
;
Elias, Juliana Bernardes
;
Stracieri, Ana Beatriz P. L.
;
Pieroni, Fabiano
;
Cunha, Renato
;
Darrigo-Junior, Luiz Guilherme
;
Settani Grecco, Carlos Eduardo
;
Covas, Dimas Tadeu
;
Silva-Pinto, Ana Cristina
;
De Santis, Gil Cunha
;
Simoes, Belinda Pinto
;
Oliveira, Maria Carolina
;
Toubert, Antoine
;
Ribeiro Malmegrim, Kelen Cristina
Total Authors: 21
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| Document type: | Journal article |
| Source: | CLINICAL & TRANSLATIONAL IMMUNOLOGY; v. 11, n. 4, p. 16-pg., 2022-01-01. |
| Abstract | |
Objectives. Allogeneic haematopoietic stem cell transplantation (allo-HSCT) is the only currently available curative treatment for sickle cell disease (SCD). Here, we comprehensively evaluated the reconstitution of T- and B-cell compartments in 29 SCD patients treated with allo-HSCT and how it correlated with the development of acute graft-versus-host disease (aGvHD). Methods. T-cell neogenesis was assessed by quantification of signal-joint and beta-chain TCR excision circles. B-cell neogenesis was evaluated by quantification of signal-joint and coding-joint K-chain recombination excision circles. T- and B-cell peripheral subset numbers were assessed by flow cytometry. Results. Before alloHSCT (baseline), T-cell neogenesis was normal in SCD patients compared with age-, gender- and ethnicity-matched healthy controls. Following allo-HSCT, T-cell neogenesis declined but was fully restored to healthy control levels at one year post-transplantation. Peripheral T-cell subset counts were fully restored only at 24 months post-transplantation. Occurrence of acute graft-versus-host disease (aGvHD) transiently affected T- and B-cell neogenesis and overall reconstitution of T- and B-cell peripheral subsets. B-cell neogenesis was significantly higher in SCD patients at baseline than in healthy controls, remaining high throughout the follow-up after allo-HSCT. Notably, after transplantation SCD patients showed increased frequencies of IL-10-producing B-regulatory cells and IgM(+) memory B-cell subsets compared with baseline levels and with healthy controls. Conclusion. Our findings revealed that the T- and B-cell compartments were normally reconstituted in SCD patients after alto-HSCT. In addition, the increase of IL-10-producing B-regulatory cells may contribute to improve immune regulation and homeostasis after transplantation. (AU) | |
| FAPESP's process: | 13/08135-2 - CTC - Center for Cell-Based Therapy |
| Grantee: | Dimas Tadeu Covas |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 16/11544-0 - Evaluation of the Thymic Function in patients with sickle cell anemia following hematopoietic stem cell transplantation and conventionally treated |
| Grantee: | Luciana Ribeiro Jarduli |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |