Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root

Author(s)

Arruda, Bruna ; Rodrigues, Yasmin Florentino ; Herrera, Wilfrand Ferney Bejarano ; Robin, Agnes ; Cotta, Simone Raposo ; Andreote, Fernando Dini

• Document type: Journal article
• Source: Journal of Microbiological Methods; v. 192, p. 4-pg., 2022-01-01.

Abstract

Mycorrhizal colonization of roots is traditionally evaluated by empirical methods, such as root microscopy. We compared this method with data from using a real time PCR technique, and determined the correlation between methods, indicating particularities of a promising system for a quick and accurate molecular diagnostic of arbuscular mycorrhization. (AU)

• FAPESP's process: 16/21596-7 - Interaction between phosphate fertilizer and soil microbial diversity in the plant colonization by arbuscular mycorrhizal fungi
• Grantee: Bruna Arruda
• Support Opportunities: Scholarships in Brazil - Doctorate