Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis

Author(s)

Maringolo-Ribeiro, Camila ; Grecco, Julia A. ; Bellato, Debora L. ; Almeida, Aryadne L. ; Baldin, Vanessa P. ; Caleffi-Ferracioli, Katiany R. ; Pavan, Fernando R.

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 17, n. 7, p. 17-pg., 2022-03-23.

Abstract

Aim: Antibiotic resistance is one of the biggest threats to global health, and this study aimed better understand how the efflux pumps are related to this process in tuberculosis clinical isolates. Results: The combination of antibiotics plus efflux pumps (EP) inhibitors was able to restore the susceptibility of clinical isolates in 100% of aminoglycosides resistance and 33.3% of the fluoroquinolones resistance. The relative expression of EP genes in pre-extensively drug-resistant isolates showed an increase of up to 1000-times. Conclusion: The rescue of susceptibility in the presence of EP inhibitors, the increased of activity and expression of the EP genes alert that the inhibition of EP can reduce the selection of resistant strains and improve treatment. (AU)

• FAPESP's process: 18/02344-2 - Evaluation of in vitro and in vivo potential of siderophores-functionalized liposomes containing moxifloxacin against Mycobacterium tuberculosis
• Grantee: Camila Maríngolo Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/12135-1 - Efflux pumps as a possible mechanism of resistance to 2nd line drugs: phenotypic and genotypic evaluation in resistant clinical isolates of Mycobacterium tuberculosis
• Grantee: Júlia Araújo Grecco
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/13497-4 - Search for the mechanism of action and therapeutic effect of new classes of drugs against Mycobacterium tuberculosis
• Grantee: Fernando Rogério Pavan
• Support Opportunities: Regular Research Grants