Effect of retained placenta and clinical mastitis on reproduction parameters, immune response, and steroidogenic receptors gene expression in postpartum crossbred dairy cows

Author(s)

Ramos, Oglenia Pereira ; Rezende, Amanda Lima ; de Alvarenga, Paula Batista ; Campos, Carla Cristian ; de Rezende, Estevao Vieira ; Barbosa Silva, Marcelo Jose ; Carneiro, Luisa Cunha ; de Moraes, Giovanna Faria ; Elsen Saut, Joao Paulo ; dos Santos, Ricarda Maria

• Document type: Journal article
• Source: TROPICAL ANIMAL HEALTH AND PRODUCTION; v. 54, n. 3, p. 11-pg., 2022-06-01.

Abstract

The purpose of this study was to evaluate the effect of retained placenta (RP) and clinical mastitis (CM) on the reproductive efficiency of crossbred dairy cows during the postpartum period and the effect in some innate immune system indicators. For this, two experiments were carried out. In the first, a total of 232 cows were evaluated and divided as: healthy control (n = 184), RP (n = 22), and CM (n = 26) groups. The RP and CM was evaluated until 30 days postpartum (DPP) and reproductive rates were measured. In experiment 2, cows were divided in control (n = 10), RP (n = 10), and CM (n = 30) groups. Between 40 and 50 DPP, clinical, gynecological examination and endometrial cytobrush were performed to evaluate subclinical endometritis (SE) and gene expression of interleukins 1 beta (IL-1 beta) and 6 (IL-6), chemokine ligand 5 (CCL5), estrogen alpha (ESR1), and progesterone (PGR) receptors by qRT-PCR analysis. In experiment 1, the conception rate at 1st artificial insemination (AI) was lower in RP and CM groups and pregnancy rate at 150 days decreased in CM group. Calving-to-1st AI interval and days open were shorter in healthy cows. In experiment 2, the occurrence of SE was 26.7% and higher in RP and CM groups. The expression of IL-1 beta increased in RP and CM groups, while IL-6 was less expressed in RP group. The CCL5, ESR1, and PGR were similar between groups. In conclusion, cows with RP and CM had their reproductive efficiency negatively affected and had they initial pro-inflammatory response improved by the increase of IL-beta. (AU)

• FAPESP's process: 18/16051-7 - Mechanisms of Omega-3 fatty acid -induced immune response in cows by in vitro cell culture and metabolic functions by in vivo supplementation
• Grantee: Luisa Cunha Carneiro
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor