| Full text | |
| Author(s): |
Calori, Italo Rodrigo
;
Alves, Samara Rodrigues
;
Bi, Hong
;
Tedesco, Antonio Claudio
Total Authors: 4
|
| Document type: | Journal article |
| Source: | ACS APPLIED BIO MATERIALS; v. 5, n. 2, p. 11-pg., 2022-02-21. |
| Abstract | |
three-dimensional culture models that resemble and mimic the complexity of the dense and hypoxic cancer microenvironment. However, in brain tumor studies, a variety of glioblastoma multiforme (GBM) cell lines only self-assemble into loose cellular aggregates, lacking the properties of actual glioma tumors in humans. In this study, we used type-I collagen as an extracellular matrix component to promote the compaction of GBM aggregates forming tight spheroids to understand how collagen influences the properties of tumors, such as their growth, proliferation, and invasion, and collagenase to promote collagen degradation. The GBM cell lines U87MG, T98G, and A172, as well as the medulloblastoma cell line UW473, were used as standard cell lines that do not spontaneously self-assemble into spheroids, and GBM U251 was used as a self-assembling cell line. According to the findings, all cell lines formed tight spheroids at collagen concentrations higher than 15.0 mu g mL(-1). Collagen was distributed along the spheroid, similarly to that observed in invasive GBM tumors, and decreased cell migration with no effect on the cellular uptake of small active molecules, as demonstrated by uptake studies using the photosensitizer verteporfin. The enzymatic cleavage of collagen affected spheroid morphology and increased cell migration while maintaining cell viability. Such behaviors are relevant to the physiological models of GBM tumors and are useful for better understanding cell migration and the in vivo infiltration path, drug screening, and kinetics of progression of GBM tumors. (AU) | |
| FAPESP's process: | 20/04507-6 - HYDROGENLS CONFINED IN 3D PRINTED MATRIX TO CONTROLLED AND SITE-SPECIFIC DELIVERY OF INGENOL-3-DODECANOATE AND FLUOROCHROME FOR PHOTODIAGNOSTIC AND THERAPY OF CEREBRAL TUMORS |
| Grantee: | Italo Rodrigo Calori |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders |
| Grantee: | Antonio Claudio Tedesco |
| Support Opportunities: | Research Projects - Thematic Grants |