Bisphenol A exposure induces multiple effects in DOPC membrane models

Author(s)

Maximino, Mateus D. ; Martin, Cibely S. ; Alessio, Priscila

• Document type: Journal article
• Source: JOURNAL OF MOLECULAR LIQUIDS; v. 359, p. 9-pg., 2022-08-01.

Abstract

Due to its now well-known endocrine-disrupting behavior, Bisphenol A (BPA) has focused on many interactions, detection, and removal studies in the last few years. The application of simple membrane models to identify specific interactions in some regions of the cells, such as the membrane, is essential to help understand the aspects of the mechanisms of action. This work evaluated the effects of the BPA exposure over membrane models of a fluid phospholipid, the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The BPA interaction with the DOPC monolayers was performed using Langmuir films, evaluating changes in pi-A isotherms, lipid fluidity, and interaction at the molecular level by PM-IRRAS spectroscopy. Giant unilamellar vesicles (GUVs) were used to reveal alteration in the shape and morphology of the lipid bilayers promoted by BPA. Multiple effects were observed in the GUVs, including surface fluctuations, an endocytosis-like effect, the formations of budding, and scissions. These alterations could be related to changes observed in the phosphate groups of DOPC in the PM-IRRAS analysis and the lipophilic properties of BPA. Furthermore, the changes observed indicate that BPA has an affinity to the membrane, which could lead to different implications or effects in cells not yet understood, which requires further investigations. (C) 2022 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/15019-0 - Combination of confocal fluorescence microscopy and surface-enhanced Raman scattering techniques: interaction, detection, and quantification of molecules of interest in biomimetic system.
• Grantee: Cibely da Silva Martin Sonvesso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/14235-6 - Membrane mimetic models applied in interaction studies, detection, and removal of emerging pollutants
• Grantee: Priscila Alessio Constantino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/06534-8 - Impact of Emerging Pollutants in Health: a Study on Biomimetic Membranes
• Grantee: Priscila Alessio Constantino
• Support Opportunities: Regular Research Grants