Microcystin-LR at sublethal concentrations induce rapid morphology of liver and muscle tissues in the fish species Astyanax altiparanae (Lambari)

Author(s)

Rodrigues, Natali Bosque ; Pitol, Dimitrius Leonardo ; de Figueiredo, Fellipe Augusto Tocchini ; Lima, Ana Claudia Tenfen das Chagas ; Henry, Theodore Burdick ; Issa, Joao Paulo Mardegan ; Umbuzeiro, Gisela de Aragao ; Pereira, Bruno Fiorelini

• Document type: Journal article
• Source: Toxicon; v. 211, p. 9-pg., 2022-05-01.

Abstract

The process of eutrophication and consequent proliferation of cyanobacteria in rivers and lakes leads to increasing numbers of harmful algal blooms and higher concentration of toxic metabolites in freshwater bodies. Microcystin is a toxic metabolite produced by cyanobacteria that is frequently detected and can pose health risks to important freshwater species including fish. The aim of the present study was to evaluate the effects of microcystin-LR on the morphology of Astyanax altiparanae's liver and muscle. One hundred (n = 100) Astyanax altiparanae were divided into 5 groups (n = 20) with 24 h and 96 h of microcystin exposition at two doses of 0.5 and 1.0 mu g/L. Differences were observed in the microcystin treatment with respect to histopathological analyses including cytoplastic degradation, displacement, and increase in nuclei volume and area of hepatocytes. Hyperemia and dilation of blood capillaries were seen in the liver. There were also observable changes in the size of muscle fibers and muscle inflammation. Our results demonstrate that microcystins can impact the integrity of both tissues even at sublethal concentrations. Low doses of microcystins are therefore sufficient to intoxicate fish livers and muscle tissues. (AU)

• FAPESP's process: 16/02946-7 - Morphological identification of biomarkers for the microcystin toxin in fish species Astyanax altiparanae
• Grantee: Dimitrius Leonardo Pitol
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/24438-1 - Toxic effects of microcystin-LR on white musculature of Astyanax altipanae fish
• Grantee: Nátali Bosquê Rodrigues
• Support Opportunities: Scholarships in Brazil - Scientific Initiation