| Full text | |
| Author(s): |
Ruas, Juliana S.
;
Siqueira-Santos, Edilene S.
;
Navarro, Claudia D. C.
;
Castilho, Roger F.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | DATA IN BRIEF; v. 40, p. 11-pg., 2022-02-01. |
| Abstract | |
Determination of oxygen consumption is one of the most valuable methodologies to evaluate mitochondrial (dys)function. Previous studies demonstrated that a widely used protocol, consisting of adding the ATP synthase inhibitor oligomycin before mitochondrial respiratory uncoupling by sequential addition of a protonophore (e.g., carbonyl cyanide 3-chlorophenyl hydrazone [CCCP]), may lead to underestimation of maximal oxygen consumption rate (OCR max ) and spare respiratory capacity (SRC) parameters in highly glycolytic tumor cell lines. In this dataset, we report the effects of the glycolytic inhibitors 2-deoxy-D-glucose, iodoacetic acid, and lonidamine on overcoming the underestimation of OCR max and SRC in oligomycin-treated cells. We propose a protocol in which 2-deoxy-D-glucose is added after oligomycin and just before the sequential addition of CCCP to avoid underestimation of OCR max and SRC parameters in A549, C2C12, and T98G cells. The oxygen consumption rates were determined in intact suspended cell lines using a highresolution oxygraph device. The data can be used in several fields of research that require characterization of mitochondrial respiratory parameters in intact cells. (c) 2021 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) (AU) | |
| FAPESP's process: | 19/20855-7 - The role of mitochondrial NAD(P)-Transhydrogenase in the development of Fatty Liver Disease and aging in mice |
| Grantee: | Claudia Daniele Carvalho Navarro |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |