The role of mitochondrial NAD(P)-Transhydrogenase in the development of Fatty Liver Disease and aging in mice

• Grant number: 19/20855-7
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): November 01, 2019
• Effective date (End): October 31, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal researcher: Aníbal Eugênio Vercesi
• Grantee: Claudia Daniele Carvalho Navarro
• Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/17728-8 - Mitochondrial function and dysfunction: implications for aging and associated diseases, AP.TEM

Abstract

We will study the role of mitochondrial NAD(P)-transhydrogenase (NNT) activity in the development of Fatty Liver Disease and aging in mice. It will be investigated the role of pyruvate dehydrogenase inhibition in the development of Fatty Liver Disease in NNT deficient mice fed a high fat diet. Additionally, the involvement of NNT activity will be evaluated in the process of mitochondrial permeability transition pore opening and aging-related mitochondrial redox imbalance. Methodological approaches will include: maintenance of a mouse colony including performing genetic control and administration of diets and drugs, evaluation of histological aspects of Fatty Liver Disease, assessment of the bioenergetic and redox balance in isolated mitochondria, blood cell isolation, flow cytometry, and measurements of glycolytic rate and oxygen uptake in adherent cells. (AU)