Infection by coffee ringspot virus is identified for the first time in succulent plants

Author(s)

Nunes, Maria Andreia ; Ferreira, Leticia Maria ; Rogerio, Luana Aparecida ; Kitajima, Elliot Watanabe ; Novelli, Valdenice Moreira

• Document type: Journal article
• Source: JOURNAL OF PLANT PATHOLOGY; v. 104, n. 2, p. 5-pg., 2022-03-23.

Abstract

Coffee ringspot virus (CoRSV) belongs to the Dichorhavirus genus and has been considered the causal agent of the emergent disease in commercial coffee orchards. The virus is commonly transmitted by Brevipalpus papayensis Baker (Acari: Tenuipalpidae) in a persistent propagative mode, however B. yothersi is another mite vector. Here, we report for the first time that the common succulent panda plant (Kalanchoe tomentosa Baker) and bear's paw (Cotyledon tomentosa Harv.) can be infected with CoRSV transmitted by viruliferous B. papayensis females. Leaves showed typical symptoms of chlorotic and necrotic spots seven days after inoculation and RT-PCR confirmed the presence of CoRSV in symptomatic tissues. Amplicon sequences showed > 97% identity with a known CoRSV sequence (Genbank accession number GQ979998). In addition, transmission electron microscopy revealed the presence of typical dichorhavirus particles in the nucleus of symptomatic tissues of panda plants. Thus, considering the relevance of succulents in the ornamental market and the emergent CoRSV, it is important to provide information about the effects of this virus in different hosts. (AU)

• FAPESP's process: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/25078-9 - Genomics and trascriptome of virus-vector-host plant relationship in the pathosytem of Brevipalpus transmitted viruses; systematic and evolution of Brevipalpus mites and their endosymbionts; new strategies to manage citrus Leprosis in the S. Paulo State
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/23464-8 - Interaction citrus leprosis virus (CiLV-C) and different Brevipalpus species
• Grantee: Leticia Maria Ferreira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation