| Full text | |
| Author(s): |
Cynthia Aparecida Castro
;
Tereza Cristina Buzinari
[2]
;
Rafael Luis Bressani Lino
[3]
;
Heloisa Sobreiro Selistre de Araújo
[4]
;
Fernanda de Freitas Aníbal
[5]
;
Roberto Mario Machado Verzola
[6]
;
Vanderlei Salvador Bagnato
[7]
;
Natalia Mayumi Inada
[8]
;
Gerson Jhonatan Rodrigues
[9]
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Cardiologia; 2022-08-05. |
| Abstract | |
Abstract Background The formation of foam cells occurs due to the increase in low-density plasma lipoprotein (LDL) and dysregulation of inflammation, which is important for the development of atherosclerosis. Objective To evaluate the profile of tumor necrosis factor-alpha (TNF-α) and Interleukin-6 (IL-6) in the existing foam cell formation method, optimizing this protocol. Methods The LDL was isolated, oxidized, and labeled with a Fluorescein isothiocyanate (FITC) probe. Foam cells were generated from THP-1 human monocyte-derived cells and incubated in the absence (control) or presence of FITC-ox-LDL (10, 50, 100, 150, or 200 μg/mL), for 12, 24, 48, or 72 hours. The accumulated FITC-ox-LDL in the cell was quantified by microscopy. The enzyme-linked immunosorbent assay was evaluated to quantify the IL-6 and TNF-α, with p < 0.05 considered significant. Results All the FITC-ox-LDL concentrations tested showed a higher fluorescence when compared to the control, showing a greater accumulation of lipoprotein in cells. The higher the concentration of FITC-ox-LDL, the greater the production of TNF-α and IL-6. The production of IL-6 by foam cells was detected up to the value of 150 µg/mL of the maximum stimulus for LDL. Concentrations above 50 μg/mL LDL stimulated greater release of TNF-α compared to control. Conclusions Our model contributes to the understanding of the release of IL-6 and TNF-α in response to different concentrations of ox-LDL, using an optimized method for the formation of foam cells. (AU) | |
| FAPESP's process: | 18/10588-9 - Characterization of the hypotensive effect by photobiomodulation in different hypertension animal models and pharmacological strategies to enhance this effect |
| Grantee: | Gerson Jhonatan Rodrigues |
| Support Opportunities: | Regular Research Grants |