Rheological study of PEG-Laponite-alginate composite hydrogels aiming 3D extrusion-based printing

Author(s)

Karina Feliciano Santo ; José Luis Dávila ^[2] ; Marcos Akira d'Ávila ^[3] ; Andrea Cecília Dorion Rodas ^[4] ; Jorge Vicente Lopes da Silva ^[5] ; Juliana Kelmy Macário Barboza Daguano

• Document type: Journal article
• Source: MATERIA-RIO DE JANEIRO; v. 27, n. 2 2022-12-05.

Abstract

ABSTRACT Hydrogels, crosslinked polymeric networks capable of absorbing and retaining a large number of water due to their hydrophilic nature, draws attention to Tissue Engineering applications. These materials offer the potential to design scaffolds based on their pseudoplastic behavior, which is essential for extrusion-based three-dimensional (3D) printing. The addition of Laponite, a two-dimensional disk-shaped nanosilicate, allows modifying the rheological behavior of some gels, creating an optimized condition for 3D printing. In this study, the rheological characterization of the composite hydrogel PEG-Laponite-Alginate (PL-Alg) and its precursor gel PEG-Laponite (PL) was carried out, aiming in the future for 3D bioprinting of constructs. Initially, the rheological behavior of different concentrations of Laponite in the PL composite was evaluated. The viscosity of the PEG-400 increased dramatically as a function of the addition of Laponite, characterizing a strong shear-thinning and solid-like rheological behavior, ideal for 3D printing. The effect of different levels of Laponite can also be noted for the composite (PL-Alg), keeping the concentration of PEG400 and alginate fixed, the latter being added as a second network precursor to crosslink the PEG-Laponite hydrogel. Besides, all composites showed a partial recovery of viscosity as a function of time after the application of shear. This rheological parameter must be considered in the development of a bioink. PL-Alg scaffolds containing Laponite were printed containing up to 10 layers and placed in a CaCl2 solution to promote crosslinking of the alginate chains. Subsequently, in vitro tests were performed in phosphate-buffered saline solution, showing that the dissolution of the composite hydrogel network was more critical for the sample with 5% Laponite. In conclusion, for future work, the most appropriate way of crosslinking the PEG chain should be considered to improve the mechanical properties and the resistance to degradation, enabling the use of the PEG-Laponite-Alginate system as a bioink for 3D bioprinting applications. (AU)

• FAPESP's process: 19/11950-6 - Biofabrication studies for osteochondral structure
• Grantee: Jorge Vicente Lopes da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/23776-5 - Rheology and Additive Manufacturing of Nanocomposite Hydrogels for Applications in Regenerative Medicine
• Grantee: Marcos Akira d'Ávila
• Support Opportunities: Regular Research Grants