Morphological and molecular characterization of Hysterothylacium spp. parasitizing Pomatomus saltatrix and Pagrus pagrus of the State of São Paulo, Brazil

Author(s)

THAISSA D. SERRANO ^[1] ; DIEGO H.M.D. VIEIRA ^[2] ; LARISSA S. PELEGRINI ^[3] ; LÚCIA V. FRAGOSO ^[4] ; BEATRIZ N. AGOSTINHO ^[5] ; MANUEL VERA ^[6] ; FÁBIO PORTO-FORESTI ^[7] ; RODNEY K. DE AZEVEDO ^[8] ; VANESSA D. ABDALLAH ^[9]

Affiliation

^[1] Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP). Instituto de Biociências de Botucatu - Brasil

^[2] Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP). Instituto de Biociências de Botucatu - Brasil

^[3] Universidade Federal do Amazonas (UFAM). Laboratório de Ictiologia e Ordenamento Pesqueiro do Vale do Rio Madeira - Brasil

^[4] Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP). Instituto de Biociências de Botucatu - Brasil

^[5] Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP). Instituto de Biociências de Botucatu - Brasil

^[6] University of Santiago de Compostela (USC). Faculty of Veterinary - Espanha

^[7] Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP). Faculdade de Ciências - Brasil

^[8] Centro Universitário CESMAC. Programa de Pós-Graduação em Análise de Sistemas Ambientais - Brasil

^[9] Universidade Federal de Alagoas (UFAL). Instituto de Ciências Biológicas e da Saúde - Brasil

• Document type: Journal article
• Source: Anais da Academia Brasileira de Ciências; v. 95, n. 1 2023-02-10.

Abstract

Abstract Raphidascarid nematodes have been the focus of several studies, mainly due to the zoonotic potential of some species, even though the cases are underreported. Due to the difficulty in identifying their larvae, the use of diagnostic techniques involving morphological and molecular analyses has grown in the last 20 years. The present study had as objective the morphological and molecular characterization of the L3 larval types of Hysterothylacium collected in Pomatomus saltatrix and Pagrus pagrus from the Brazilian coast, close to the municipality of Santos, State of São Paulo. Twenty specimens of P. saltatrix were necropsied and Hysterothylacium type V (n = 257) and Hysterothylacium type X (n = 5) larvae were found. Five specimens of P. pagrus were necropsied and all were parasitized by Hysterothylacium type V larvae. The analyses showed a genetic proximity relationship between Hysterothylacium types V with other Hysterothylacium V and with H. deardorffoverstreetorum, although this is a species inquirenda. Haplotypes for Hysterothylacium type X found in the present study formed a monophyletic group with other Hysterothylacium X, H. amoyense, and H. zhoushanense. Through this study, new hosts and localities were registered for Hysterothylacium type V and Hysterothylacium type X. (AU)

• FAPESP's process: 17/14332-6 - Molecular study of anisakid nematodes collected in Pomatomus saltatrix used in Japanese food preparation
• Grantee: Thaíssa Duarte Serrano
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 16/00513-6 - Taxonomic study and molecular of anisakid nematodes collected in three fish species used in preparation of Japanese food
• Grantee: Thaíssa Duarte Serrano
• Support Opportunities: Scholarships in Brazil - Master