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Taxonomic study and molecular of anisakid nematodes collected in three fish species used in preparation of Japanese food

Grant number: 16/00513-6
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2017
Effective date (End): February 28, 2018
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Marine Fishing Resources
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Vanessa Doro Abdallah Kozlowiski
Grantee:Thaíssa Duarte Serrano
Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil
Associated scholarship(s):17/14332-6 - Molecular study of anisakid nematodes collected in Pomatomus saltatrix used in Japanese food preparation, BE.EP.MS


Fish are one of the most important protein sources of animal origin for human consumption, with high digestibility and satisfactory levels of unsaturated fats, vitamins and minerals. Currently restaurants specializing in Japanese food have become common in Brazil and concern with the introduction of culinary habits involved in the spread of parasitic diseases transmitted by fish is present. Among these diseases, there is the anisakiasis, which can occur through ingestion of meat treated raw or undercooked fish containing the third or fourth stage larvae and in this case the man acts as an accidental host and the larvae did not complete their development. These parasites can enter the digestive tract of humans and invade the attached organs causing a number of pathological effects. Larvae anisakid are resistant to salting, smoking and similar procedures and, for this reason, the preparation of fish for human consumption requires the use of temperatures higher than 60 ° C for at least ten minutes. For any parasitological survey and epidemiological study to be conducted with these parasites, the exact identification of the species occurring in a geographical area, is the first parameter to be considered. Based on the above, were selected for this study, the species of marine fish Pomatomus saltratix and Micropogonias furnieri and the species freshwater Oreochromis niloticus whose muscles, stomach, intestine and mesentery will be analyzed to verify the possible presence of nematodes with zoonotic potential, as such species of fish are widely used raw in the preparation of sushi and sashimi. The results of this study may help health authorities, fish farmers and veterinarians to pay attention to more control, from production to the marketing of fish to reduce the morbidity and mortality of creations, better and better the quality of the fish for the consumer and prophylactically, to prevent the spread of zoonoses transmitted by fish. (AU)