| Full text | |
| Author(s): |
Fabio C Dias
[1]
;
Kerlei C Médici
[2]
;
Bruna Alexandrino
[3]
;
Andréa S.R Medeiros
[4]
;
Amauri A Alfieri
[5]
;
Samir I Samara
[6]
Total Authors: 6
|
| Affiliation: | [1] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Medicina Veterinária Preventiva e Reprodução Animal - Brasil
[2] Universidade Estadual de Londrina. Centro de Ciências Agrárias. Departamento de Medicina Veterinária Preventiva - Brasil
[3] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Medicina Veterinária Preventiva e Reprodução Animal - Brasil
[4] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Medicina Veterinária Preventiva e Reprodução Animal - Brasil
[5] Universidade Estadual de Londrina. Centro de Ciências Agrárias. Departamento de Medicina Veterinária Preventiva - Brasil
[6] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Medicina Veterinária Preventiva e Reprodução Animal - Brasil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 30, n. 11, p. 933-939, 2010-11-00. |
| Abstract | |
The research on persistently infected (PI) animals with bovine viral diarrhoea virus (BVDV) was conducted in 26 cattle herds, which were not BVDV vaccinated, located in the states of Minas Gerais and São Paulo, Brazil. Using a sampling strategy, five samples of blood were collected from 6 to 12-month-old calves of each herd, and the blood sera were tested by virusneutralization test (VN) to BVDV-1 and BVDV-2. The herds that had at least three out of five samples reacting to one of the genotypes of BVDV and antibody titers greater than 128 were selected to PI animals research. In three of the herds that matched the before-mentioned criteria, paired blood samples were collected from all its individuals considering a collection interval of 30 days. The blood sera of these samples were VN tested against BVDV-1 and BVDV-2. In samples not reacting to at least one of the BVDV genotypes and also in those collected from calves of less than six months of age, virus research was undertaken by reverse transcription polymerase chain reaction (RT-PCR). From the examined herds, two PI animals were detected in paired samples obtained from a herd located in the state of Minas Gerais. (AU) | |