Genotyping of Toxoplasma gondii strains detected in pork sausage

ARISTEU VIEIRA DA SILVA; ANDRÉ DE OLIVEIRA MENDONÇA; SANDIA BERGAMASCHI PEZERICO; PAULO FRANCISCO DOMINGUES; HELIO LANGONI

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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genotyping of Toxoplasma gondii strains detected in pork sausage

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Author(s):	ARISTEU VIEIRA DA SILVA ; ANDRÉ DE OLIVEIRA MENDONÇA ^[2] ; SANDIA BERGAMASCHI PEZERICO ^[3] ; PAULO FRANCISCO DOMINGUES ^[4] ; HELIO LANGONI ^[5] Total Authors: 5
Document type:	Journal article
Source:	Parasitol. latinoam.; v. 60, p. 65-68, 2005-06-00.
Abstract
DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments in the city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples. Digestion of amplicons with restriction enzymes HhaI and Sau3AI showed that 14 (73.68%) samples contained parasites genotyped as Type I, and 5 (26.32%) as Type III (AU)

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