| Full text | |
| Author(s): Show less - |
Telles, Guilherme Defante
;
Libardi, Cleiton Augusto
;
Conceicao, Miguel Soares
;
Vechin, Felipe Cassaro
;
Lixandrao, Manoel Emilio
;
Rotea Mangone, Flavia Regina
;
Pavanelli, Ana Carolina
;
Nagai, Maria Aparecida
;
Camera, Donny Michael
;
Hawley, John A.
;
Ugrinowitsch, Carlos
Total Authors: 11
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| Document type: | Journal article |
| Source: | MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 55, n. 2, p. 10-pg., 2023-02-01. |
| Abstract | |
IntroductionDNA methylation regulates exercise-induced changes in the skeletal muscle transcriptome. However, the specificity and the time course responses in the myogenic regulatory factors DNA methylation and mRNA expression after divergent exercise modes are unknown.PurposeThis study aimed to compare the time course changes in DNA methylation and mRNA expression for selected myogenic regulatory factors (MYOD1, MYF5, and MYF6) immediately after, 4 h after, and 8 h after a single bout of resistance exercise (RE), high-intensity interval exercise (HIIE), and concurrent exercise (CE).MethodsNine healthy but untrained males (age, 23.9 +/- 2.8 yr; body mass, 70.1 +/- 14.9 kg; peak oxygen uptake [V?O-2peak], 41.4 +/- 5.2 mL center dot kg(-1)center dot min(-1); mean +/- SD) performed a counterbalanced, randomized order of RE (4 x 8-12 repetition maximum), HIIE (12 x 1 min sprints at V?O-2peak running velocity), and CE (RE followed by HIIE). Skeletal muscle biopsies (vastus lateralis) were taken before (REST) immediately (0 h), 4 h, and 8 h after each exercise bout.ResultsCompared with REST, MYOD1, MYF5, and MYF6, mean methylation across all CpGs analyzed was reduced after 4 and 8 h in response to all exercise protocols (P < 0.05). Reduced levels of MYOD1 methylation were observed after HIIE and CE compared with RE (P < 0.05). Compared with REST, all exercise bouts increased mRNA expression over time (MYOD1 at 4 and 8 h, and MYF6 at 4 h; P < 0.05). MYF5 mRNA expression was lower after 4 h compared with 0 h and higher at 8 h compared with 4 h (P < 0.05).ConclusionsWe observed an interrelated but not time-aligned response between the exercise-induced changes in myogenic regulatory factors demethylation and mRNA expression after divergent exercise modes. Despite divergent contractile stimuli, changes in DNA methylation and mRNA expression in skeletal muscle were largely confined to the late (4-8 h) recovery period and similar between the different exercise challenges. (AU) | |
| FAPESP's process: | 18/12150-0 - CONCURRENT TRAINING: EFFECT ON EPIGENETIC MECHANISM OF DNA METHILATION |
| Grantee: | Cleiton Augusto Libardi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/16513-0 - The effects of concurrent training on the epigenetic mechanism of DNA methylation |
| Grantee: | Guilherme Defante Telles |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 17/01297-8 - Effects of concurrent training on the epigenetic mechanism of DNA methylation |
| Grantee: | Guilherme Defante Telles |
| Support Opportunities: | Scholarships in Brazil - Master |