Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training

Author(s)

Scariot, P. P. M. ; Manchado-Gobatto, F. B. ; Beck, W. R. ; Papoti, M. ; Van Ginkel, P. R. ; Gobatto, C. A.

• Document type: Journal article
• Source: Life Sciences; v. 307, p. 11-pg., 2022-08-11.

Abstract

Aims: The synthesis of monocarboxylate transporters (MCTs) can be stimulated by aerobic training, but few is known about this effect associated or not with non-voluntary daily activities. We examined the effect of eight weeks of aerobic training in MCTs on the skeletal muscle and hypothalamus of less or more physically active mice, which can be achieved by keeping them in two different housing models, a small cage (SC) and a large cage (LC). Main methods: Forty male C57BL/6J mice were divided into four groups. In each housing condition, mice were divided into untrained (N) and trained (T). For 8 weeks, the trained animals ran on a treadmill with an intensity equivalent to 80 % of the individual critical velocity (CV), considered aerobic capacity, 40 min/day, 5 times/ week. Protein expression of MCTs was determined with fluorescence Western Blot. Key findings: T groups had higher hypothalamic MCT2 than N groups (ANOVA, P = 0.032). Significant correlations were detected between hypothalamic MCT2 and CV. There was a difference between the SC and LC groups in relation to MCT4 in the hypothalamus (LC > SC, P = 0.044). Trained mice housed in LC (but not SC-T) exhibited a reduction in MCT4 muscle (P < 0.001). Significance: Our findings indicate that aerobically trained mice increased the expression of MCT2 protein in the hypothalamus, which has been related to the uptake of lactate in neurons. Changes in energy metabolism in physically active mice (kept in LC) may be related to upregulation of hypothalamic MCT4, probably participating in the regulation of satiety. (AU)

• FAPESP's process: 18/07099-6 - High intensity interval training (HIIT) in running: effects on aerobic and anaerobic capacity, stress biomarkers, bone and muscle responses in active and sedentary mice
• Grantee: Emanuel Elias Camolese Polisel
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/10201-4 - Effects of aerobic physical training and the availability of wide space housing on the spontaneous physical activity, central and peripheral metabolic and molecular responses
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/05115-7 - Effects of aerobic training and the hypoxic environment on molecular responses related to anaerobic and aerobic metabolism and acid-base properties in the hypothalamus and skeletal muscle of mice
• Grantee: Pedro Paulo Menezes Scariot
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/01362-9 - Effects of age, caloric restriction diet (Cr) and SIRT3 on spontaneous activity, behavior and oxidative mediators (NO, NOS and orexin) and hypothalamic mitochondrial respiration
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 15/00272-6 - Effects of caloric restriction mediated by SIRT3 in the aerobic and anaerobic capacities of mice: relations with the spontaneous activity, metabolic parameters and muscle mitochondrial function in aging process
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 14/10336-9 - Effect of different training models with load control over physiological parameters, genic expressions and protein contents of HIF-1 alpha, PGC-1 alpha, MCT1 and MCT4: relationship with performance and spontaneous activity of swimming rats
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Regular Research Grants