The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer

Author(s)

Dias, Luana Mendonca ; Klein, Marlise Inez ; Ferrisse, Tulio Morandin ; Medeiros, Karine Sousa ; Jordao, Claudia Carolina ; Bellini, Amanda ; Pavarina, Ana Claudia

• Document type: Journal article
• Source: JOURNAL OF FUNGI; v. 9, n. 1, p. 22-pg., 2023-01-01.

Abstract

This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine (R) (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L-; C+L-), and LED (P-L+; C-L+). Control groups were used (P-L-; C-L-). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed similar to 1.40 log(10) increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ. (AU)

• FAPESP's process: 18/14874-6 - Potential of antimicrobial photodynamic therapy in the development of resistance in Candida albicans
• Grantee: Luana Mendonça Dias Santana
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/27634-6 - Evaluation effect of DNAse-associated antimicrobial photodynamic therapy in the treatment of Oral Candidosis in mice infected with Candida albicans
• Grantee: Cláudia Carolina Jordão
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/01191-0 - Tolerance induction and Candida albicans's biofilm characterization after antimicrobial photodymamic therapy application
• Grantee: Túlio Morandin Ferrisse
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC