Determination of selected herbicides employed in sugarcane crops by disposable pipette tip (DPX) extraction followed by LC-MS/MS and GC-MS/MS

Author(s)

Oliveira, Thais Camarotto ; Lancas, Fernando Mauro

• Document type: Journal article
• Source: Journal of Liquid Chromatography & Related Technologies; v. 44, n. 17-20, p. 8-pg., 2022-08-22.

Abstract

A disposable pipette tip extraction (DPX) method followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed and validated to determine eleven herbicides employed in sugarcane crops. After improving conditions, the DPX method consists of conditioning a C18 tip with 0.3 mL of methanol, followed by the same volume of water and 0.15 mL of the sample. After completing the sorption interaction, the sample was dispensed, and elution was made with 0.15 mL of methanol before collecting the extract for injection. The total extraction time was 4 min, and recoveries ranged from 59.1 to 76.5%. DPX method was compared to cartridge solid-phase extraction, which presented recoveries between 30.4 and 108.6%. SPE consumed four times more solvent, total extraction time is five times longer than DPX, and cartridges are more expensive than the DPX tips. DPX performance was also evaluated by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The recoveries were similar to LC-MS/MS. Furthermore, the DPX-C18-LC-MS/MS method was applied to analyze sugarcane-derived juice samples. Results indicate that the method can determine the herbicides in actual samples and other sugarcane matrices. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/07347-9 - Development of novel materials and devices for miniaturized sample preparation techniques in the area of food safety
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry
• Grantee: Deyber Arley Vargas Medina
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/15462-5 - Employment of graphene as sorbent in miniaturized sample preparation techniques.
• Grantee: Bruno Henrique Fumes
• Support Opportunities: Scholarships in Brazil - Doctorate