miR-29c Increases Protein Synthesis in Skeletal Muscle Independently of AKT/mTOR

Author(s)

Nascimento Alves, Paula Ketilly ; Cruz, Andre ; Silva, William J. ; Labeit, Siegfried ; Moriscot, Anselmo Sigari

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 13, p. 11-pg., 2022-07-01.

Abstract

microRNAs negatively regulate gene expression by blocking translation or increasing mRNA degradation. In skeletal muscle, these molecules play important roles in adaptive responses, and ongoing investigations are necessary to understand the fine-tune regulation of skeletal muscle mass. Herein we showed that skeletal muscle overexpression of miR-29c increased fiber size and force at 7 and 30 days after electrotransfer. At both time points, AKT/mTOR pathway components were downregulated, and, surprisingly, overall protein synthesis was strongly elevated at day 7, which normalized by day 30 after pCMVmiR-29c electrotransfer. These results indicate that miR-29c expression induces skeletal muscle hypertrophy and gain of function, which involves increased overall protein synthesis in spite of the deactivation of the AKT/mTOR pathway. (AU)

• FAPESP's process: 21/05827-7 - Effect of leucine on the expression of miR-299a and HDAC4 in the skeletal muscle of rats submitted to hind limb immobilization: implications for the control of muscle mass
• Grantee: Paula Ketilly Nascimento Alves
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/08996-4 - Effects of miR-29c on skeletal muscle plasticity: implications for the control of muscle mass
• Grantee: William Jose da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/24419-4 - Effect of leucine on the expression of miR-299A and HDAC4 in the skeletal muscle of rats submitted to immobilization: implications for the control of muscle mass
• Grantee: Paula Ketilly Nascimento Alves
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/04090-0 - Identification and caracterization of mechanisms involved in skeletal muscle mass control and regeneration
• Grantee: Anselmo Sigari Moriscot
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/09398-8 - Interplay betweeen myostatin and mTORC1 pathways in skeletal muscle: implications for a thyroid hormone biological action
• Grantee: André Cruz de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/03066-9 - Skeletal muscle mass control by microRNA-29c manipulation: a molecular intervention approach
• Grantee: Anselmo Sigari Moriscot
• Support Opportunities: Regular Research Grants