MALDI-TOF Mass Spectrometry for the Diagnosis of Citrus Canker Caused by Xanthomonas citri subsp. citri

Author(s)

Niculau, Edenilson dos Santos ; Ferreira, Douglas ; Rodrigues-Filho, Edson ; Behlau, Franklin ; Magnani, Rodrigo Facchini ; Toffano, Leonardo ; Prieto, Evandro Luis ; Fernandes, Joao Batista ; da Silva, Maria Fatima das Gracas Fernandes

• Document type: Journal article
• Source: Molecules; v. 27, n. 24, p. 14-pg., 2022-12-01.

Abstract

Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a disease that causes serious problems to the global citrus industry. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS) has been used in human medicine to diagnose various diseases caused by both fungi and bacteria. In agriculture, this technique has potential for the diagnosis of diseases due to the low cost of large-scale analysis and quickness. This study showed that MALDI-TOF MS combined with chemometric analysis was effective for differentiating the macromolecule profile of orange leaves with canker lesions, healthy leaves, and leaves with phytotoxicity symptoms, proving that this technique may be used for the rapid diagnosis of citrus canker. (AU)

• FAPESP's process: 08/57859-5 - Biorrational control of insect pests
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/22975-8 - Chemical study of Xanthomonas axonopodis pv. citri and its influence on chemical profile of Citrus sinensis
• Grantee: Edenilson dos Santos Niculau
• Support Opportunities: Scholarships in Brazil - Doctorate