Preventive effect of chitosan gel containing CaneCPI-5 against enamel erosive wear in situ

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Pela, Vinicius Taioqui ; Brito, Leonardo ; Taira, Even Akemi ; Henrique-Silva, Flavio ; Pieretti, Joana Claudio ; Seabra, Amedea Barozzi ; Baldini Cardoso, Cristiane de Almeida ; de Souza, Eduardo Pereira ; Groisman, Sonia ; Rodrigues, Marcela Charantola ; Lussi, Adrian ; Carvalho, Thiago Saads ; Rabelo Buzalaf, Marilia Afonso

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 11, p. 9-pg., 2022-07-13.

Abstract

Objective This study evaluated the preventive effect of a chitosan gel containing CaneCPI-5 against enamel erosion and erosion + abrasion in situ. Methods Sixteen volunteers participated in a crossover, double-blind protocol, comprising 4 phases: (1) no treatment (Nt); (2) chitosan gel (Cg); (3) chitosan gel + 12,300 ppm NaF (Cg + NaF); and (4) chitosan gel + 0.1 mg/mL CaneCPI-5 (Cg + Cane). Volunteers wore an appliance containing 4 specimens. Once/day, they applied the gel (except for Nt) (4 min/specimen). Erosive challenges were performed extra-orally (0.1% citric acid, 90 s, 4 x /day; ERO). Specimens were also abraded (toothbrush, 15 s/specimen, 2 x /day; ERO + ABR). Enamel wear was assessed by profilometry and relative surface reflection intensity (%SRI). Two-way RM-ANOVA/Sidak's tests and Spearman's correlation were used (p < 0.05). Results For profilometry, ERO + ABR promoted significantly greater wear when compared with ERO. There was a significant difference among all treatments. The lowest enamel loss occurred for Cg + Cane, followed by Cg + NaF, Cg, and Nt (p < 0.05). The %SRI was significantly lower for ERO + ABR when compared to ERO, only for the Nt group. The greatest %SRI was found for the Cg + NaF and Cg + Cane groups, which did not differ significantly, regardless of the conditions. The lowest %SRI was found for the Nt and Cg groups, which did not differ from each other, regardless of the conditions. The Nt group did not differ significantly from the Cg + NaF (ERO). There was a significant correlation between both analyses. Conclusion The incorporation of CaneCPI-5 in the chitosan gel prevented erosive wear in situ. (AU)

• FAPESP's process: 20/03646-2 - Impact of nanoplatforms allied to nitric oxide and chemotherapeutics on cytotoxicity and sensitization of resistant tumor cells
• Grantee: Joana Claudio Pieretti
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/04857-4 - Acquired pellicle engineering for control of dental erosion: in situ evaluation of protective potential by a new cistatin derived from sugar cane
• Grantee: Vinícius Taioqui Pelá
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/02366-9 - Acquired pellicle engineering with CaneCPI-5 added to a topically applied gel against dentin erosion: in situ study
• Grantee: Leonardo Brito
• Support Opportunities: Scholarships in Brazil - Scientific Initiation