| Full text | |
| Author(s): Show less - |
Ferreira, Savio Siqueira
;
Goeminne, Geert
;
Simoes, Marcella Siqueira
;
de Almeida Pina, Andre Vicioli
;
Alves de Lima, Leydson Gabriel
;
Pezard, Jade
;
Gutierrez, Ana
;
Rencoret, Jorge
;
Mortimer, Jenny C.
;
del Rio, Jose C.
;
Boerjan, Wout
;
Cesarino, Igor
Total Authors: 12
|
| Document type: | Journal article |
| Source: | Journal of Experimental Botany; v. 73, n. 18, p. 27-pg., 2022-07-05. |
| Abstract | |
Phenolic metabolism is rewired during internode development and in response to cinnamyl alcohol dehydrogenase perturbation in sorghum, with different sets of genes and accumulating compounds. The molecular mechanisms associated with secondary cell wall (SCW) deposition in sorghum remain largely uncharacterized. Here, we employed untargeted metabolomics and large-scale transcriptomics to correlate changes in SCW deposition with variation in global gene expression profiles and metabolite abundance along an elongating internode of sorghum, with a major focus on lignin and phenolic metabolism. To gain deeper insight into the metabolic and transcriptional changes associated with pathway perturbations, a bmr6 mutant [with reduced cinnamyl alcohol dehydrogenase (CAD) activity] was analyzed. In the wild type, internode development was accompanied by an increase in the content of oligolignols, p-hydroxybenzaldehyde, hydroxycinnamate esters, and flavonoid glucosides, including tricin derivatives. We further identified modules of genes whose expression pattern correlated with SCW deposition and the accumulation of these target metabolites. Reduced CAD activity resulted in the accumulation of hexosylated forms of hydroxycinnamates (and their derivatives), hydroxycinnamaldehydes, and benzenoids. The expression of genes belonging to one specific module in our co-expression analysis correlated with the differential accumulation of these compounds and contributed to explaining this metabolic phenotype. Metabolomics and transcriptomics data further suggested that CAD perturbation activates distinct detoxification routes in sorghum internodes. Our systems biology approach provides a landscape of the metabolic and transcriptional changes associated with internode development and with reduced CAD activity in sorghum. (AU) | |
| FAPESP's process: | 16/06917-1 - Transcriptomic analysis and co-expression network construction in model systems for identification of genes involved in secondary cell wall deposition and lignin metabolism in grasses |
| Grantee: | Sávio de Siqueira Ferreira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/02527-1 - Development of model systems in sorghum and a systems biology approach to unravel the molecular mechanisms controlling lignin metabolism in grasses |
| Grantee: | Igor Cesarino |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |
| FAPESP's process: | 19/14604-1 - The ABC of lignification: towards the identification of ABC transporters potentially involved in lignin deposition in Setaria viridis (L.) P.Beauv |
| Grantee: | Leydson Gabriel Alves de Lima |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 15/18361-5 - Establishment of an inducible suspension cell culture for tracheary element differentiation in Sorghum bicolor L. |
| Grantee: | Marcella Siqueira Simões |
| Support Opportunities: | Scholarships in Brazil - Master |