Metabolism-epigenetic interactions on in vitro produced embryos

Author(s)

Milazzotto, Marcella Pecora ; Ispada, Jessica ; de Lima, Camila Bruna

• Document type: Journal article
• Source: REPRODUCTION FERTILITY AND DEVELOPMENT; v. N/A, p. 14-pg., 2022-10-14.

Abstract

Metabolism and epigenetics, which reciprocally regulate each other in different cell types, are fundamental aspects of cellular adaptation to the environment. Evidence in cancer and stem cells has shown that the metabolic status modifies the epigenome while epigenetic mechanisms regulate the expression of genes involved in metabolic processes, thereby altering the metabolome. This crosstalk occurs as many metabolites serve as substrates or cofactors of chromatin-modifying enzymes. If we consider the intense metabolic dynamic and the epigenetic remodelling of the embryo, the comprehension of these regulatory networks will be important not only for understanding early embryonic development, but also to determine in vitro culture conditions that support embryo development and may insert positive regulatory marks that may persist until adult life. In this review, we focus on how metabolism may affect epigenetic reprogramming of the early stages of development, in particular acetylation and methylation of histone and DNA. We also present other metabolic modifications in bovine embryos, such as lactylation, highlighting the promising epigenetic and metabolic targets to improve conditions for in vitro embryo development. (AU)

• FAPESP's process: 19/25982-7 - Metabolic regulation of DNA methylation: new routes, new targets
• Grantee: Marcella Pecora Milazzotto
• Support Opportunities: Regular Research Grants