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Development of structures based on carbon nanotubes, bioresorbable polymers and carbon fibers for studies with regenerative cells and hematopoietic stem cells

Grant number: 08/11642-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2009
Effective date (End): March 31, 2011
Field of knowledge:Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
Principal Investigator:Evaldo Jose Corat
Grantee:Anderson de Oliveira Lobo
Home Institution: Instituto Nacional de Pesquisas Espaciais (INPE). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). São José dos Campos , SP, Brazil
Associated research grant:07/00013-4 - New materials, studies and applications of CVD diamond, diamond-like-carbon (DLC) and nanostructured carbon obtained by chemical vapor deposition, AP.TEM


Nanostructured biomaterials are very promising because present good similarity with natural nanostructure components of the extracellular matrix, arousing great interest in the field of bioengineering. Its spatial configurations and the physical-chemical influence on cellular interactions that lead to the regeneration of tissues are seen as a great potential in implantable surfaces. In the classes of nanostructured materials the multi-walled carbon nanotubes (MWCNT) have great potential for biomedical applications, due to their unique properties such as high electrical conductivity, high chemical stability, high mechanical strength and facilitation of functional groups incorporation for scaffolds production. For this new material incorporation in biomedical applications, it is necessary research on its toxicity and biocompatibility in vitro and in vivo, to evaluate its ability and performance in biological systems. The objective of this work will be to analyze the biocompatibility in vitro and in vivo; and signaling possibility for hematopoietic steam cell migrations for the acceleration of regenerative cell therapy aimed at coatings and surfaces of implantable pieces. CNT / biorreabsorble polymer and carbon fiber/CNT nanocomposites are subjects of this research. The CNT nanocomposites will be produced at the Associated Laboratory of Sensors and Materiais (LAS/INPE). The biocompatibility in vitro (direct and indirect cytotoxicity, cellular adhesion and immunohistochemistry analysis) and in vivo will be carried through in the Laboratory of Dinamic and Compartments of Cells of the UNIVAP and Multidisciplinary Center for Biological Research in Area of Science in Laboratory Animals (CEMIB) of the UNICAMP, respectively. Permanent fibroblast cell lines of rats (L-929), human osteoblasts (SaOS-2) and mouse embryos fibroblasts cells extracted for C57BL/6 mice will be used for the in vitro tests. For the in vivo tests it will be performed implants of the CNT nanocomposites in mice (C57BL/6) transplanted with bone marrow cells labeled with GFP protein. We expect to prove the biocompatibility and in vitro regenerative cells proliferation on CNT/bioresorbable polymer scaffolds, and the acceleration of the regenerative process through hematopoietic stem cell recruitment and migration in implanted regions with CNT scaffolds. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LOBO, A. O.; CORAT, M. A. F.; ANTUNES, E. F.; RAMOS, S. C.; PACHECO-SOARES, C.; CORAT, E. J. Cytocompatibility studies of vertically-aligned multi-walled carbon nanotubes: Raw material and functionalized by oxygen plasma. Materials Science & Engineering C-Materials for Biological Applications, v. 32, n. 4, p. 648-652, MAY 1 2012. Web of Science Citations: 17.
RAMOS, S. C.; LOBO, A. O.; DE VASCONCELOS, G.; ANTUNES, E. F.; TRAVA-AIROLDI, V. J.; CORAT, E. J. Influence of polar groups on the wetting properties of vertically aligned multiwalled carbon nanotube surfaces. THEORETICAL CHEMISTRY ACCOUNTS, v. 130, n. 4-6, p. 1061-1069, DEC 2011. Web of Science Citations: 10.
MACHADO, SUSANE MOREIRA; LOBO, ANDERSON OLIVEIRA; LOUREIRO SAPUCAHY, ARIEL BUENO; MARCIANO, FERNANDA ROBERTA; CORAT, EVALDO JOSE; DA SILVA, NEWTON SOARES. Tritrichomonas foetus adhere to superhydrophilic vertically aligned multi-walled carbon nanotube surface. Materials Science & Engineering C-Materials for Biological Applications, v. 31, n. 7, p. 1614-1617, OCT 10 2011. Web of Science Citations: 3.
RAMOS, S. C.; VASCONCELOS, G.; ANTUNES, E. F.; LOBO, A. O.; TRAVA-AIROLDI, V. J.; CORAT, E. J. CO(2) laser treatment for stabilization of the superhydrophobicity of carbon nanotube surfaces. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, v. 28, n. 6, p. 1153-1157, NOV 2010. Web of Science Citations: 17.
LOBO, ANDERSON O.; ANTUNES, ERICA F.; PALMA, MARIANA B. S.; PACHECO-SOARES, CRISTINA; TRAVA-AIROLDI, VLADIMIR J.; CORAT, EVALDO J. Monolayer formation of human osteoblastic cells on vertically aligned multiwalled carbon nanotube scaffolds. Cell Biology International, v. 34, n. 4, p. 393-398, APR 2010. Web of Science Citations: 8.
RAMOS‚ SC; VASCONCELOS‚ G.; ANTUNES‚ EF; LOBO‚ AO; TRAVA-AIROLDI‚ VJ; CORAT‚ EJ. Total re-establishment of superhydrophobicity of vertically-aligned carbon nanotubes by Co< sub> 2 laser treatment. SURFACE & COATINGS TECHNOLOGY, v. 204, n. 18, p. 3073-3077, 2010.
LOBO, A. O.; CORAT, M. A. F.; ANTUNES, E. F.; PALMA, M. B. S.; PACHECO-SOARES, C.; CORAT, E. J. Cytotoxicity analysis of vertically aligned multi-walled carbon nanotubes by colorimetric assays. Synthetic Metals, v. 159, n. 21-22, SI, p. 2165-2166, NOV 2009. Web of Science Citations: 5.

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