Fungal communities in Brazilian cassava tubers and food products

Author(s)

Ono, L. T. ; Silva, J. J. ; Soto, T. S. ; Dona, S. ; Iamanaka, B. T. ; Fungaro, M. H. P. ; Taniwaki, M. H.

• Document type: Journal article
• Source: International Journal of Food Microbiology; v. 384, p. 10-pg., 2023-01-02.

Abstract

Cassava (Manihot esculenta Crantz) is one of the most widely cultivated foods in the world and is of great socioeconomic importance, especially in developing countries. It is predominantly consumed in boiled form, but also is used to produce a number of products, including cassava starch, sour starch, cassava flour and tapioca flour (hydrated cassava starch). Fungal spoilage can occur throughout the production chain, impairing both productivity and quality, as well as posing a potential risk of contamination by mycotoxins. We used multidisciplinary approaches based on phenotypic and molecular data (ITS/BenA/TEF-1a/RPB2 loci) to investigate the mycobiota of 101 samples (including roots, soil and products) collected in the state of Sa similar to o Paulo, Brazil. A total of 20 fungal groups/genera were morphologically characterized, and 37 different species were molecularly identified. The predominant groups in cassava tubers were Fusarium spp., Penicillium spp. and Trichoderma spp. In cassava products, the most frequent groups were Penicillium spp. and Paecilomyces spp. Potentially toxigenic species were also found, including Paecilomyces saturatus, Penicillium citrinum, P. paneum, P. brevicompactum, P. chrysogenum, Fusarium foetens and Fusarium mundagurra. In soil-cultivated cassava samples, the groups found most frequently were Penicillium spp., Cladosporium spp. and Fusarium spp. Some of the species found in cassava tubers and/or product samples were also present in the soil, including F. mundagurra, Neocosmospora solani, P. citrinum and P. brevicompactum. In general, there was a higher occurrence of Penicillium spp., Fusarium spp. and Trichoderma spp., and the predominant species were F. fabacearum and P. citrinum. The mycobiota of Brazilian cassava proved to be extremely diverse, and the occurrence of several species in cassava tubers and/or products are reported herein for the first time. Potentially toxigenic species were found in cassava tubers, cassava products and soil, showing how important it is to constantly monitor these substrates. (AU)

• FAPESP's process: 18/25552-0 - Prospecting the genetic diversity of Aspergillus section Flavi isolated in Brazil
• Grantee: Beatriz Thie Iamanaka
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/05414-8 - Isolation, identification and development of molecular marks for evaluation of Aspergillus niger producers of fumonisin B2 and ochratoxin A
• Grantee: Beatriz Thie Iamanaka
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/25597-3 - Prospecting the genetic diversity of Aspergillus section Flavi isolated in Brazil
• Grantee: Josué José da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral