| Full text | |
| Author(s): |
Bertin, Jessica S. F.
;
Marques, Maria Julia
;
Macedo, Aline B.
;
de Carvalho, Samara C.
;
Neto, Humberto S.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | JOURNAL OF MANIPULATIVE AND PHYSIOLOGICAL THERAPEUTICS; v. 45, n. 2, p. 7-pg., 2022-02-01. |
| Abstract | |
Objective: The purpose of this study was to investigate whether photobiomodulation (PBM) can protect against and attenuate muscle atrophy owing to complete peripheral nerve lesion in mice by acting on autophagy. Methods: C57BL/10 mice underwent right sciatic nerve transection to induce tibialis anterior muscle atrophy. After 6 hours of denervation, the mice received PBM (wavelength, 830 nm) daily, transcutaneously over the tibialis anterior muscle region for 5 or 14 days. Some mice with sciatic nerve lesion did not receive PBM. Mice that did not have sciatic nerve lesion and PBM were used as controls. After 5 and 14 days, the right tibialis anterior muscle was examined using histomorphometric (cross-sectional area of muscle fibers), Western blot (levels of the autophagy marker LC3), and immunofluorescence analyses (number of LC3 puncta in the muscle fibers). Results: The cross-sectional area of the tibialis anterior muscle fibers decreased after 5 and 14 days of denervation. PBM protected against muscle fiber atrophy after 5 days of denervation and attenuated muscle fiber atrophy after 14 days of denervation. After 5 days of muscle denervation, autophagy did not change, as demonstrated by the comparable levels of LC3-I/II ratio and LC3 puncta between the controls and the mice with atrophic muscle; PBM did not change this profile. After 14 days of denervation, an increased LC3-I/II ratio suggested an ongoing autophagy, which was not affected by PBM. Conclusion: PBM attenuated the tibialis anterior muscle atrophy induced by sciatic nerve transection in the mice after at least 5 and 14 days of muscle denervation, without affecting autophagy. The transient protective effect of PBM was observed as early as 5 days after the of complete nerve lesion. (AU) | |
| FAPESP's process: | 17/24051-4 - G-protein coupled receptors and autophagy: potential targets of omega-3 and deflazacort in DMD therapy |
| Grantee: | Maria Julia Marques |
| Support Opportunities: | Regular Research Grants |