| Full text | |
| Author(s): Show less - |
Nishiyama-Jr, Milton Y.
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Tashima, Alexandre K.
;
Nishiduka, Erika S.
;
Abreu, Thiago F.
;
Abukawa, Fernanda Midori
;
Oliveira, Ursula C.
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Tardivo, Caio E. O.
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Nascimento, Soraia M.
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Meissner, Gabriel O.
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Chaim, Olga M.
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Juliano, Maria A.
;
Kitano, Eduardo S.
;
Zelanis, Andre
;
Serrano, Solange M. T.
;
da Silva Jr, Pedro I.
;
Junqueira-de-Azevedo, Inacio L.
Total Authors: 16
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| Document type: | Journal article |
| Source: | JOURNAL OF PROTEOME RESEARCH; v. 21, n. 11, p. 15-pg., 2022-10-19. |
| Abstract | |
Acanthoscurria juruenicola is an Amazonian spider described for the first time almost a century ago. However, little is known about their venom composition. Here, we present a multiomics characterization of A. juruenicola venom by a combination of transcriptomics, proteomics, and peptidomics approaches. Transcriptomics of female venom glands resulted in 93,979 unique assembled mRNA transcript encoding proteins. A total of 92 proteins were identified in the venom by mass spectrometry, including 14 mature cysteine-rich peptides (CRPs). Quantitative analysis showed that CRPs, cysteine-rich secretory proteins, metalloproteases, carbonic anhydrases, and hyaluronidase comprise >90% of the venom proteome. Relative quantification of venom toxins was performed by DIA and DDA, revealing converging profiles of female and male specimens by both methods. Biochemical assays confirmed the presence of active hyaluronidases, phospholipases, and proteases in the venom. Moreover, the venom promoted in vivo paralytic activities in crickets, consistent with the high concentration of CRPs. Overall, we report a comprehensive analysis of the arsenal of toxins of A. juruenicola and highlight their potential biotechnological and pharmacological applications. Mass spectrometry data were deposited to the ProteomeXchange Consortium via the PRIDE repository with the dataset identifier PXD013149 and via the MassIVE repository with the dataset identifier MSV000087777. (AU) | |
| FAPESP's process: | 17/20106-9 - Peptidomics of Brazilian snake and spider venoms |
| Grantee: | Alexandre Keiji Tashima |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/13588-0 - Multi - user equipment approved in grant 2012/50191-4: mass spectrometer impact II - Bruker |
| Grantee: | Maria Aparecida Juliano |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |