3D-printed beta-TCP/S53P4 bioactive glass scaffolds coated with tea tree oil: Coating optimization, in vitro bioactivity and antibacterial properties

Author(s)

Nogueira Alves, Ana Paula ; Arango-Ospina, Marcela ; Moraes Saldanha Oliveira, Rodrigo Luiz ; Ferreira, Igor Maia ; de Moraes, Elisangela Guzi ; Hartmann, Malte ; Novaes de Oliveira, Antonio Pedro ; Boccaccini, Aldo Roberto ; Triches, Eliandra de Sousa

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 111, n. 4, p. 14-pg., 2022-11-28.

Abstract

Bone infection treatment is a significant challenge for the orthopedic field. 3D printing is a promising technology to produce scaffolds with customized architecture, able to stimulate and support bone growth. beta-TCP and S53P4 bioactive glass (BG) are well-known biomaterials for scaffold manufacturing. However, a multifunctional scaffold, able to inhibit microbial proliferation at the defect site, is of increasing interest to avoid infection recurrence. Tea tree oil (TTO) has aroused interest as an antimicrobial agent to minimize the use of antibiotics. Therefore, combining the regenerative potential of a bioceramic with TTO's antimicrobial properties could result in a scaffold capable of stimulating tissue growth and treating infections. In this context, this study aimed to produce and characterize 3D-printed beta-TCP/S53P4 BG scaffolds coated with TTO. Scaffolds morphological and chemical characterizations were carried out through XDR, SEM, and FTIR analysis. beta-TCP/S53P4 BG scaffolds showed a compressive strength of similar to 2 MPa and 53 +/- 2% of porosity. The scaffolds were coated by two different procedures, using an ethanol/TTO (EtOH/TTO) and a gelatin/TTO (Gel/TTO) solution with 5, 10, and 15% (v/v) TTO. The addition of TTO decreased MG-63 cell viability for both coating groups, but the Gel/TTO group showed higher cell viability. The antibacterial activity of the coated scaffolds was evaluated against S. aureus and higher inhibition of colony formation was found for Gel/TTO group. Therefore, the coating with Gel/TTO was effective in terms of antibacterial activity and cell viability. Such Gel/TTO coated beta-TCP/S53P4 BG scaffolds are proposed for antibacterial bone tissue engineering. (AU)

• FAPESP's process: 19/19187-0 - Production of S53P4 bioglass/sodium alginate scaffolds using the robocasting technique: antimicrobial activity evaluation and paste rheological optimization
• Grantee: Rodrigo Luiz Moraes Saldanha Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/08141-9 - B-TCP/BG S53P4 ceramic pastes rheological parameters optimization to scaffolds production by robocasting method
• Grantee: Ana Paula Nogueira Alves
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/19594-4 - 3D pritting of bioactive and resorbable scaffolds by robocasting: synthesis, processing and antibacterial activity
• Grantee: Eliandra de Sousa Trichês
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/12944-7 - Evaluation of antibacterial and biological activity of B-TCP/S53P4 scaffolds obtained by robocasting and coated by tea tree oil
• Grantee: Ana Paula Nogueira Alves
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree