| Full text | |
| Author(s): |
Perles, Livia
;
Herrera, Heitor M.
;
Barreto, Wanessa T. G.
;
de Macedo, Gabriel C.
;
Calchi, Ana C.
;
Machado, Rosangela Z.
;
Andre, Marcos R.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | MICROORGANISMS; v. 10, n. 12, p. 15-pg., 2022-12-01. |
| Abstract | |
The Anaplasmataceae family encompasses obligate intracellular alpha-proteobacteria of human and veterinary medicine importance. This study performed multi-locus sequencing to characterize Ehrlichia and Anaplasma in coati's blood samples in Midwestern Brazil. Twenty-five samples (25/165-15.1%) were positive in the screening PCR based on the dsb gene of Ehrlichia spp. and were characterized using 16S rRNA, sodB, groEL, and gltA genes and the 23S-5S intergenic space region (ITS). Phylogenetic analyses based on all six molecular markers positioned the sequences into a new clade, with a common origin of Ehrlichia ruminantium. Haplotype analyses of 16S RNA sequences revealed the presence of two distinct Ehrlichia genotypes. Six samples (6/165, 3.6%) were positive in the screening nPCR for the 16S rRNA gene of Anaplasma spp. and were submitted to an additional PCR targeting the ITS for molecular characterization. Phylogenetic analyses based on both 16S rRNA gene and ITS positioned the Anaplasma sp. detected in the present study in a large clade with other Anaplasma sp. previously detected in ticks and wild animals and in a clade with 'Candidatus Anaplasma brasiliensis', respectively. Based on distinct molecular markers, the present work described a putative novel Anaplasmataceae agent, namely 'Candidatus Ehrlichia dumleri', and Anaplasma sp. closely related to the previously described 'Candidatus Anaplasma brasiliensis'. (AU) | |
| FAPESP's process: | 20/12037-0 - Diversity of piroplasmids in wild birds and mammals from Brazil |
| Grantee: | Marcos Rogério André |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/02753-0 - ISOLATION AND GENOTYPING OF Bartonella spp. IN DOMESTIC AND WILD RESERVOIR MAMMALS IN BRAZIL |
| Grantee: | Marcos Rogério André |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/15150-4 - Genetic diversity of vector-borne agents in coatis (Nasua nasua) in a periurban region of Central-Western Brazil |
| Grantee: | Lívia Perles |
| Support Opportunities: | Scholarships in Brazil - Doctorate |