Production, Physicochemical and Structural Characterization of a Bioemulsifier Produced in a Culture Medium Composed of Sugarcane Bagasse Hemicellulosic Hydrolysate and Soybean Oil in the Context of Biorefineries

Author(s)

Barbosa, Fernanda Goncalves ; Marcelino, Paulo Ricardo Franco ; Lacerda, Talita Martins ; Philippini, Rafael Rodrigues ; Giancaterino, Emma Teresa ; Mancebo, Marcos Campos ; dos Santos, Julio Cesar ; da Silva, Silvio Silverio

• Document type: Journal article
• Source: FERMENTATION-BASEL; v. 8, n. 11, p. 21-pg., 2022-11-01.

Abstract

Biosurfactants are amphipathic molecules, biodegradable, with reduced toxicity. They can be synthesized by fermentative processes from oleaginous compounds and agro-industrial by-products. In this context, the present study describes the production and the physical, chemical, and structural characterization of the bioemulsifier secreted by the yeast Scheffersomyces shehatae 16-BR6-2AI in a medium containing hemicellulosic sugarcane bagasse hydrolysate combined with soybean oil. The bioemulsifier was produced in Erlenmeyer flasks and isolated; then, the physicochemical and structural characterization of the formed molecule was carried out. The following fermentation parameters were obtained: Y-X/S = 0.45, Y-P/S = 0.083, and productivity of 0.076 g/L/h. The bioemulsifier was found to be a polymer containing 53% of carbohydrates, 40.92% of proteins, and 6.08% of lipids, respectively. The FTIR spectrum confirmed the presence of functional groups such as amides, amines, and carbonyls. The bioemulsifier was stable over a range of temperature (-20 degrees C to 120 degrees C), salinity (1-15%), and pH (2-12). It was observed that the biomolecule has a better emulsifying action in organic solvents with a non-polar character. Therefore, this biomolecule is a potential substitute for synthetic surfactants and can be used in different applications. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 16/14852-7 - A sustainable solution against neglected tropical diseases using biosurfactants produced by yeasts in sugarcane bagasse hemicellulosic hydrolyzate
• Grantee: Paulo Ricardo Franco Marcelino
• Support Opportunities: Scholarships in Brazil - Post-Doctoral