| Full text | |
| Author(s): Show less - |
Hespanhol, Julia Takuno
;
Sanchez-Limache, Daniel Enrique
;
Nicastro, Gianlucca Goncalves
;
Mead, Liam
;
Llontop, Edgar Enrique
;
Chagas-Santos, Gustavo
;
Farah, Chuck Shaker
;
de Souza, Robson Francisco
;
Galhardo, Rodrigo da Silva
;
Lovering, Andrew L.
;
Bayer-Santos, Ethel
Total Authors: 11
|
| Document type: | Journal article |
| Source: | eLIFE; v. 11, p. 26-pg., 2022-10-13. |
| Abstract | |
The type VI secretion system (T6SS) secretes antibacterial effectors into target competitors. Salmonella spp. encode five phylogenetically distinct T6SSs. Here, we characterize the function of the SPI-22 T6SS of Salmonella bongori showing that it has antibacterial activity and identify a group of antibacterial T6SS effectors (TseV1-4) containing an N-terminal PAAR-like domain and a C-terminal VRR-Nuc domain encoded next to cognate immunity proteins with a DUF3396 domain (TsiV1-4). TseV2 and TseV3 are toxic when expressed in Escherichia coli and bacterial competition assays confirm that TseV2 and TseV3 are secreted by the SPI-22 T6SS. Phylogenetic analysis reveals that TseV1-4 are evolutionarily related to enzymes involved in DNA repair. TseV3 recognizes specific DNA structures and preferentially cleave splayed arms, generating DNA double-strand breaks and inducing the SOS response in target cells. The crystal structure of the TseV3:TsiV3 complex reveals that the immunity protein likely blocks the effector interaction with the DNA substrate. These results expand our knowledge on the function of Salmonella pathogenicity islands, the evolution of toxins used in biological conflicts, and the endogenous mechanisms regulating the activity of these toxins. (AU) | |
| FAPESP's process: | 17/17303-7 - Structure and function of bacterial secretion systems |
| Grantee: | Shaker Chuck Farah |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/12234-2 - Isolation and structural studies on the Xanthomonas citri Type IV pilus and T4SS pilus |
| Grantee: | Edgar Enrique Llontop Cornejo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 21/03400-6 - Development of a universal pipeline for genomic context analysis from different sources of data. |
| Grantee: | Gianlucca Gonçalves Nicastro |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 16/09047-8 - Comparative genomics of bacterial toxins associated with type IV secretion systems |
| Grantee: | Robson Francisco de Souza |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/15389-4 - Cloning and antibacterial activity of novel Salmonella T6SS effectors |
| Grantee: | Gustavo Chagas Santos |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 18/25316-4 - Function of SPI-6 T6SS and SPI-19 T6SS of Salmonella during competition with the gut microbiota |
| Grantee: | Julia Takuno Hespanhol |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 18/04553-8 - Function of type VI secretion systems of pathogenic bacteria in the interaction with eukaryotic cells |
| Grantee: | Ethel Bayer Santos |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 17/02178-2 - Function of type VI secretion systems of pathogenic bacteria in the interaction with eukaryotic cells |
| Grantee: | Ethel Bayer Santos |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 19/22715-8 - Function of Salmonella bongori SPI-22 T6SS in bacterial competitions and interactions with phagocytic host cells |
| Grantee: | Daniel Enrique Sánchez Limache |
| Support Opportunities: | Scholarships in Brazil - Master |